Human CD48/SLAMF2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD48/SLAMF2 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) OX-40, rhCD84, or recombinant mouse CD48.
  • Source
    Monoclonal Mouse IgG2B Clone # 394620
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD48/SLAMF2
    Gln27-Ser220
    Accession # P09326.2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CD48/SLAMF2 (Catalog # 3644-CD) under non-reducing conditions only
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD48/SLAMF2 in Human PBMCs. CD48/SLAMF2 was detected in immersion fixed non-stimulated human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human CD48/SLAMF2 Mono­clonal Antibody (Catalog # MAB36441) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
CD48/SLAMF2 in Human PBMCs. CD48/SLAMF2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD48/SLAMF2 Mono­clonal Antibody (Catalog # MAB36441) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD48/SLAMF2

CD48 is a GPI-linked protein in the CD2 subfamily of immunoglobulin superfamily proteins. Human and mouse CD48 share 51% amino acid sequence identity. CD48 binds with high affinity to the natural killer (NK) cell activating ligand 2B4 and with lower affinity to CD2. A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients.

  • Long Name:
    SLAM Family Member 2
  • Entrez Gene IDs:
    962 (Human); 12506 (Mouse); 245962 (Rat)
  • Alternate Names:
    BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2
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