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Human CD57 Antibody

Catalog #: MAB11708 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB11708-100
MAB11708-SP
Detection of CD57 in PBMC by Flow Cytometry
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Human CD57 Antibody Summary

Species Reactivity
Human
Specificity
Detects the CD57 carbohydrate epitope in Human PBMCs
Source
Monoclonal Mouse IgG2B Clone # 1088014
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Whole Cell
Accession # Q9P2W7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human Peripheral Blood Mononuclear Cells (PBMCs)
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human lymph node, spleen and tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD57 in PBMC by Flow Cytometry PBMC were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry View Larger

Detection of CD57 in Human Lymph Node. CD57 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

Detection of CD57 in Human Spleen. CD57 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

Detection of CD57 in Human Tonsil. CD57 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD57

CD57 is a glycoprotein with a molecular weight of approximately 100 kDa, classified within the group of adhesion molecules. It plays a significant role in immune regulation and cellular interactions, particularly influencing the functioning of natural killer (NK) cells and certain T-cells. CD57 is heavily involved in the biological processes of cytotoxic activity and immune response modulation. Altered levels of CD57 expression have been associated with several pathological conditions, including chronic infections, autoimmune diseases, and various malignancies. Crucially, CD57 serves as a key biomarker for immune senescence and the monitoring of NK cell and T-cell activities in diseases such as HIV and cancer.

References
1. Kared H, Martelli S, Ng TP, Pender SL, Larbi A. CD57 in human natural killer cells and T-lymphocytes. Cancer Immunol Immunother. 2016 Apr;65(4):441-52. doi: 10.1007/s00262-016-1803-z. Epub 2016 Feb 5. PMID: 26850637; PMCID: PMC11029668. 
2. Elias Junior E, Gubert VT, Bonin-Jacob CM, Puga MAM, Gouveia CG, Sichinel AH, Tozetti IA. CD57 T cells associated with immunosenescence in adults living with HIV or AIDS. Immunology. 2024 Jan;171(1):146-153. doi: 10.1111/imm.13707. Epub 2023 Oct 25. PMID: 37880915. 
3. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020 Nov 10;13(1):151. doi: 10.1186/s13045-020-00986-z. Erratum in: J Hematol Oncol. 2025 Mar 5;18(1):25. doi: 10.1186/s13045-025-01682-6. PMID: 33168037; PMCID: PMC7653700.
Long Name
Cluster of Differentiation 57
Entrez Gene IDs
27087 (Human); 76898 (Mouse); 117108 (Rat)
Alternate Names
CD57; HNK1; HNK-1; Human Natural Killer-1; L2; LEU7; LEU-7

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