Human CD79A Antibody Summary
Accession # P11912
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CD79A by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB6920) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CD79A at approximately 44 to 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD79A, also known as Ig alpha and MB-1, is a 44 kDa transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig-like domain in its extracellular region (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform with a 39 aa deletion in the ECD. Heterodimers of CD79A and CD79B/Ig beta associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD, human CD79A shares 57% aa sequence identity with mouse and rat CD79A.
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