Human Chitinase 3-like 1 Antibody

Catalog # Availability Size / Price Qty
AF2599
AF2599-SP
Human Chitinase 3-like 1 Antibody in Western Blot
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human Chitinase 3-like 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chitinase 3-like 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Chitinase 3-like 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 1
Tyr22-Thr383
Accession # P36222
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Chitinase 3-like 1 by Western Blot. View Larger

Detection of Human Chitinase 3-like 1 by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line treated (+) with 50 ng/mL of PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Chitinase 3-like 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Chitinase 3-like 1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Chitinase 3‑like 1 in Human Ovarian Cancer. View Larger

Chitinase 3‑like 1 in Human Ovarian Cancer. Chitinase 3‑like 1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer using Goat Anti-Human Chitinase 3‑like 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) at 15 µg/mL overnight at 4 °C. Beforeincubation with the primary antibody, tissue was subjected to heat-inducedepitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013).Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Chitinase 3‑like 1 in Human Cartilage. View Larger

Chitinase 3‑like 1 in Human Cartilage. Chitinase 3‑like 1 was detected in immersion fixed paraffin-embedded sections of human cartilage using Goat Anti-Human Chitinase 3‑like 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Chitinase 3‑like 1 by Simple WesternTM. View Larger

Detection of Human Chitinase 3‑like 1 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line treated (+) with 50 ng/mL PMA for 72 hours, loaded at 0.2 mg/mL. A specific band was detected for Chitinase 3‑like 1 at approximately 49 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Chitinase 3‑like 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 1

CHI3L1, also known as HCgp39 (human cartilage glycoprotein 39) and YKL40, is a secreted glycoprotein belonging to the family of chitinase-like lectins. These proteins are structurally related to the glycosylhydrolase family 18, but lack enzymatic activity. CHI3L1 is expressed by chondrocytes, synovial cells, macrophages and neutrophils. It has been found to play a role in down‑regulating cytokine-induced inflammatory responses. Human CHI3L1 shares 73%, 80%, 83% and 86% amino acid sequence identity with mouse, rat, bovine and porcine CHI3L1, respectively.

Entrez Gene IDs
1116 (Human); 12654 (Mouse)
Alternate Names
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

Product Datasheets

Citations for Human Chitinase 3-like 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. YKL-40 (Chitinase 3-like I) is expressed in a subset of astrocytes in Alzheimer's disease and other tauopathies
    Authors: M Querol-Vil, M Colom-Cade, J Pegueroles, C San Martín, J Clarimon, O Belbin, J Fortea, A Lleó
    J Neuroinflammation, 2017;14(1):118.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
    Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
    J. Clin. Invest, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Serum YKL-40 as a marker of liver fibrosis in patients with non-alcoholic fatty liver disease
    Sci Rep, 2016;6(0):35282.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Regulation of YKL-40 expression by corticosteroids: effect on pro-inflammatory macrophages in vitro and its modulation in COPD in vivo.
    Authors: Kunz L, Van't Wout E, van Schadewijk A, Postma D, Kerstjens H, Sterk P, Hiemstra P
    Respir Res, 2015;16(0):154.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Stem cells from trabecular meshwork home to TM tissue in vivo.
    Authors: Du Y, Yun H, Yang E, Schuman J
    Invest Ophthalmol Vis Sci, 2013;54(2):1450-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  6. The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.
    Authors: Park JA, Drazen JM, Tschumperlin DJ
    J. Biol. Chem., 2010;285(39):29817-25.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Macrophage chitinase 1 stratifies chronic obstructive lung disease.
    Authors: Agapov E, Battaile JT, Tidwell R, Hachem R, Patterson GA, Pierce RA, Atkinson JJ, Holtzman MJ
    Am. J. Respir. Cell Mol. Biol., 2009;41(4):379-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human Chitinase 3-like 1 Antibody

Average Rating: 3.5 (Based on 2 Reviews)

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Human Chitinase 3-like 1 Antibody
By Anonymous on 10/04/2018
Application: WB Sample Tested: Primary glioblastoma stem cells Species: Human

Human Chitinase 3-like 1 Antibody
By Anonymous on 12/01/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human