Human cIAP-1/HIAP-2 Antibody

  (32 citations)
(1 Review)
    
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Product Details
Citations (32)
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  • Species Reactivity
    Human
  • Specificity
    Detects human cIAP-1/HIAP-2. Does not cross-react with recombinant human cIAP-2 or XIAP.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human cIAP-1/HIAP-2
    His2-Ser618
    Accession # Q13490
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Knockout Validated
    cIAP‑1/HIAP‑2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in cIAP‑1/HIAP‑2 knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human cIAP‑1/HIAP‑2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, lystates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line were included. A specific band for cIAP‑1/HIAP‑2 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunohistochemistry
cIAP‑1/HIAP‑2 in Human Lymph Node. cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human
cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
cIAP‑1/HIAP‑2 in Human Lymphoma. cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Knockout Validated
Western Blot Shows Human cIAP‑1/HIAP‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for cIAP‑1/HIAP‑2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-1/HIAP-2

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.

  • References:
    1. Roy, N. et al. (1997) EMBO J. 23:6914.
    2. Deveraux, Q. et al. (1997) Nature 388:300.
    3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
    4. Herrera, B. et al. (2002) FEBS Letters 520:93.
  • Long Name:
    Cellular Inhibitor of Apoptosis Protein 1
  • Entrez Gene IDs:
    329 (Human); 11797 (Mouse)
  • Alternate Names:
    API1Hiap-2; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing protein 2; BIRC2; cIAP1; C-IAP1; cIAP-1; hIAP2; HIAP-2; IAP homolog B; IAP2; IAP-2; Inhibitor of apoptosis protein 2; MIHB; MIHBHIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RNF48hiap-2; TNFR2-TRAF-signaling complex protein 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth
    Authors: GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari
    Mol. Cancer Ther., 2018;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Derivatization of inhibitor of apoptosis protein (IAP) ligands yields improved inducers of estrogen receptor ? degradation
    Authors: N Ohoka, Y Morita, K Nagai, K Shimokawa, O Ujikawa, I Fujimori, M Ito, Y Hayase, K Okuhira, N Shibata, T Hattori, T Sameshima, O Sano, R Koyama, Y Imaeda, H Nara, N Cho, M Naito
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Molluscum Contagiosum Virus MC159 abrogates cIAP1-NEMO interactions and inhibits NEMO polyubiquitination
    Authors: S Biswas, JL Shisler
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
    Authors: ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores
    Cell Death Dis, 2017;8(1):e2535.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  5. Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment
    Authors: A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka
    Cell Death Differ., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Hepatitis C Virus Infection Increases c-Jun N-Terminal Kinase (JNK) Phosphorylation and Accentuates Hepatocyte Lipoapoptosis
    Authors: H Takaki, Y Akazawa, Y Kido, M Morishita, T Honda, H Shibata, S Miuma, H Miyaaki, N Taura, H Kondo, K Nakao
    Med. Sci. Monit., 2017;23(0):4526-4532.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
    Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Development of protein degradation inducers of oncogenic BCR-ABL protein by conjugation of ABL kinase inhibitors and IAP ligands
    Authors: N Shibata, N Miyamoto, K Nagai, K Shimokawa, T Sameshima, N Ohoka, T Hattori, Y Imaeda, H Nara, N Cho, M Naito
    Cancer Sci., 2017;108(8):1657-1666.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Targeted degradation of proteins localized in subcellular compartments by hybrid small molecules
    Authors: Keiichiro Okuhira
    Mol. Pharmacol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival
    Authors: L Hernandez, MK Kim, AM Noonan, E Sagher, H Kohlhammer, G Wright, LT Lyle, PS Steeg, M Anver, DD Bowtell,, CM Annunziata
    Cell Death Discov, 2015;1(0):15053.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death.
    Authors: Schoeneberger H, Belz K, Schenk B, Fulda S
    Oncogene, 2015;34(31):4032-43.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
    Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, Gregory
    PLoS ONE, 2014;9(3):e92124.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
    Authors: Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J, Zhang X, Dai S, Sun X
    J Hematol Oncol, 2014;7(0):62.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. NF-kappaB inducing kinase, a central signaling component of the non-canonical pathway of NF-kappaB, contributes to ovarian cancer progression.
    Authors: Uno M, Saitoh Y, Mochida K, Tsuruyama E, Kiyono T, Imoto I, Inazawa J, Yuasa Y, Kubota T, Yamaoka S
    PLoS ONE, 2014;9(2):e88347.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway.
    Authors: Wang Q, Chen W, Bai L, Chen W, Padilla M, Lin A, Shi S, Wang X, Lin Y
    J Biol Chem, 2014;289(9):5654-63.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  18. Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders.
    Authors: Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T
    Int J Oncol, 2014;45(1):419-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
    Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
    Anticancer Res, 2014;34(12):7007-20.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  20. Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-kappaB-dependent manner.
    Authors: Wagner L, Marschall V, Karl S, Cristofanon S, Zobel K, Deshayes K, Vucic D, Debatin K, Fulda S
    Oncogene, 2013;32(8):988-97.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  21. The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
    Authors: Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M
    Clin Cancer Res, 2013;19(7):1784-94.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  22. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
    Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
    FASEB J, 2013;28(4):1938-46.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  23. Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration.
    Authors: Oberoi-Khanuja, Tripat K, Karreman, Christia, Larisch, Sarit, Rapp, Ulf R, Rajalingam, Krishnar
    J Biol Chem, 2012;287(34):28445-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  24. Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
    Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
    PLoS ONE, 2012;7(9):e45492.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  25. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012;447(3):427-36.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  26. Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways.
    Clin. Cancer Res., 2012;18(12):3316-27.
    Species: Human
    Sample Type: Cell Lysates
    Application: Flow
  27. TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8.
    Authors: Ikner A, Ashkenazi A
    J. Biol. Chem., 2011;286(24):21546-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  28. RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis.
    Authors: Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S
    Leukemia, 2011;26(5):1020-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  29. Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade.
    Authors: Reddy RM, Yeow WS, Chua A, Nguyen DM, Baras A, Ziauddin MF, Shamimi-Noori SM, Maxhimer JB, Schrump DS
    Apoptosis, 2007;12(1):55-71.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  30. Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases.
    Authors: Balasubramanian S, Mani S, Shiraishi H, Johnston RK, Yamane K, Willey CD, Cooper G, Tuxworth WJ, Kuppuswamy D
    J. Mol. Cell. Cardiol., 2006;41(4):669-79.
    Species: Feline
    Sample Type: Tissue Homogenates
    Application: WB
  31. cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.
    Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC
    Cancer Res., 2005;65(1):210-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  32. Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications.
    Authors: Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC
    Oncogene, 2002;21(37):5673-83.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Western Blot Human cIAP-1/HIAP-2 Antibody AF8181 Array AF8181
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Western Blot Human cIAP-1/HIAP-2 Antibody AF8181
Western Blot: Human cIAP-1/HIAP-2 Antibody [AF8181].

Summary

Application Western Blot
Sample Tested MCF-7 human breast cancer cell line
Species Human

Other Experimental Details

Other Experimental Details Expression of hcIAP-1/HIAP-2 in MCF-7 cells untreated (A) and after treatment with 10 uM Paclitaxel for 4 hours (B) using Goat anti-Human cIAP-1/HIAP-2 antibody (#AF8181) at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.

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