Human CRBN Antibody

Catalog # Availability Size / Price Qty
CRBN in RAW 264.7 Mouse Cell Line.
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Human CRBN Antibody Summary

Species Reactivity
Detects human CRBN in immunocytochemistry and immunohistochemistry.
Monoclonal Mouse IgG2B Clone # 978015
Protein A or G purified from hybridoma culture supernatant
Synthetic peptide Human CRBN
Accession # Q96SW2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
1-25 µg/mL
See below
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CRBN antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

CRBN in RAW 264.7 Mouse Cell Line. CRBN was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human CRBN Polyclonal Antibody (Catalog # MAB9574) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CRBN antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

CRBN in Human Testis. CRBN was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human CRBN Polyclonal Antibody (Catalog # MAB9574) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRBN

Cerbeblon  (CRBN) is a substrate recognition component of an E3 protein ligase complex which mediates ubiquitination and proteasomal degradation of target proteins. In embryonic development, degradation of regulatory proteins is required for normal limb outgrowth. Thalidomide, a teratogenic drug prescribed to pregnant women in the 1950s, binds to CRBN in the E3 protein ligase complex. Despite its teratogenic effect, CRBN is used to treat multiple myeloma and complications of leprosy. Widely expressed, CRBN may play a role in memory and learning by regulating assembly and surface expression of large-conductance calcium-activated potassium channels in hippocampal neurons. Mutations in the CRBN gene is linked to the Mental Retardation, Autosomal Recessive2a (MR2A) form of mental retardation.

Long Name
Entrez Gene IDs
51185 (Human); 58799 (Mouse); 297498 (Rat)
Alternate Names
Cereblon; CRBN; DKFZp781K0715; MGC27358; MRT2; MRT2A; non-syndromic, autosomal recessive, 2A; protein cereblon; Protein X 0001

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Human CRBN Antibody
By Anonymous on 07/25/2022
Application: ICC/IF Sample Tested: macrophages Species: Human