Human CXCR7/RDC-1 Antibody

Catalog #: MAB4227
4 Citations Datasheet / COA / SDS

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MAB4227
MAB4227-SP

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Detection of CXCR7/RDC‑1 in Human peripheral blood Monocytes by Flow Cytometry.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR7/RDC-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types.

Source

Monoclonal Mouse IgG_2A Clone # 358426

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
Met1-Lys362 (Gly131Ser)
Accession # AAA62370

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR7/RDC‑1 in Human peripheral blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Immunohistochemistry

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CXCR7/RDC‑1 in Human Breast Cancer Tissue. CXCR7/RDC-1 was detected in perfusion fixed paraffin-embedded sections of nude mice injected with human breast cancer cells using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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CXCR7/RDC‑1 in Human Breast Cancer Tissue. CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

References

Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
Infantino, S. et al. (2006) J. Immunol. 176:2197.
Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Alternate Names

ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human CXCR7/RDC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Ligand-specific conformational transitions and intracellular transport required for atypical chemokine receptor 3-mediated chemokine scavenging
Authors: N Montpas, G St-Onge, N Nama, D Rhainds, B Benredjem, M Girard, G Hickson, V Pons, N Heveker
J. Biol. Chem., 2017-11-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
Authors: Takao T, Asanoma K, Kato K
PLoS ONE, 2011-07-07;6(7):e21990.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
Immunol. Lett., 2010-10-07;134(2):167-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CXCR7 protein is not expressed on human or mouse leukocytes.
Authors: Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ
J. Immunol., 2010-10-01;185(9):5130-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry