Human EAAT1/GLAST-1 Antibody

Catalog # Availability Size / Price Qty
EAAT1/GLAST-1 in Human Brain Tissue.
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Product Details
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Human EAAT1/GLAST-1 Antibody Summary

Species Reactivity
Detects human EAAT1/GLAST-1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 482420
Protein A or G purified from ascites
E. coli-derived recombinant human EAAT1/GLAST‑1
Accession # P43003
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry EAAT1/GLAST-1 antibody in Human Brain Tissue by Immunohistochemistry (IHC-P). View Larger

EAAT1/GLAST-1 in Human Brain Tissue. EAAT1/GLAST-1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) tissue using Mouse Anti-Human EAAT1/GLAST-1 Monoclonal Antibody (Catalog # MAB6048) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal puncta in molecular layer. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EAAT1/GLAST-1

GLAST-1 (Sodium dependent GLu/ASp Transporter 1; also SLC1A3 and EAAT1) is a 60‑62 kDa member of the SDF symporter family of molecules. It is expressed by glia, fibroblasts, and select neuron cell types such as hippocampal neurons. EAAT1 is known to transport L‑glutamate into glia, thus removing glutamate from synaptic areas where it either acts too long, or becomes toxic at elevated concentration. Its action is dependent on its ability to cotransport sodium. Human EAAT1 is a 542 amino acid (aa), 8-transmembrane variably glycosylated protein that contains N- and C-terminal cytoplasmic domains. Homodimers are reported to exist, but only when EAAT1 shows glycosylation. There are at least two potential isoform variants. One shows an Arg substitution for aa 430‑475, while a second shows a four aa substitution for aa 62‑542. Over an extracellular loop that encompasses aa 146‑237, human EAAT1 shares 92% aa identity with mouse EAAT1.

Long Name
Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1
Entrez Gene IDs
6507 (Human); 20512 (Mouse); 29483 (Rat)
Alternate Names
EA6; EA6FLJ25094; EAAT1; EAAT1GLAST-1; excitatory amino acid transporter 1; GLAST1; GLAST-1; GLASTGLAST1; SLC1A3; Sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Solute carrier family 1 member 3

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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