Human FAK Antibody

Catalog # Availability Size / Price Qty
MAB4467
MAB4467-SP
Detection of Human FAK by Western Blot.
4 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human FAK Antibody Summary

Species Reactivity
Human
Specificity
Detects human FAK in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 495919
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FAK
Asp213-Thr412
Accession # Q05397
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line,

Immunohistochemistry
8-25 µg/mL
See below
Knockout Validated
FAK is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in FAK knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FAK antibody by Western Blot. View Larger

Detection of Human FAK by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh-7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry FAK antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

FAK in Human Brain. FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human FAK Monoclonal Antibody (Catalog # MAB4467) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human FAK Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human FAK Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human FAK by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FAK at approximately 120 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAK

Focal adhesion kinase 1 (FAK), also known as FAK1 and PTK2, is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in focal adhesions. This cellular localization is directed by a C-terminal 125 amino acid "Focal Adhesion Targeting" (FAT) sequence. FAK plays an important role in migration, cell spreading, differentiation and apoptosis. It associates with several different signaling proteins, such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and Paxillin. These associations enable FAK to function within a network of integrin-stimulated signaling pathways, leading to the activation of targets such as the ERK and JNK mitogen-activated protein kinase pathways. Increased expression and/or activity of FAK in various cancers has been correlated with enhanced proliferation, migration and invasiveness of human tumor cells.

Long Name
Focal adhesion kinase 1
Entrez Gene IDs
5747 (Human); 14083 (Mouse); 25614 (Rat)
Alternate Names
EC 2.7.10; FADK 1; FADK1; FADKFAK-related non-kinase polypeptide; FAK; FAK1FRNK; FAKEC 2.7.10.2; focal adhesion kinase 1; pp125FAK; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; PTK2

Product Datasheets

You must select a language.

x

Citations for Human FAK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. A Three-Dimensional Xeno-Free Culture Condition for Wharton's Jelly-Mesenchymal Stem Cells: The Pros and Cons
    Authors: B Koh, N Sulaiman, MB Fauzi, JX Law, MH Ng, TL Yuan, AGN Azurah, MH Mohd Yunus, RBH Idrus, MD Yazid
    International Journal of Molecular Sciences, 2023-02-13;24(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Simple Western
  2. Integrin – Dependent Mechanotransduction in Mechanically Stimulated Human Annulus Fibrosus Cells: Evidence for an Alternative Mechanotransduction Pathway Operating with Degeneration
    Authors: Hamish T. J. Gilbert, Navraj S. Nagra, Anthony J. Freemont, Sarah J. Millward-Sadler, Judith A. Hoyland
    PLoS ONE

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FAK Antibody

There are currently no reviews for this product. Be the first to review Human FAK Antibody and earn rewards!

Have you used Human FAK Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review