Human Galectin-10 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Galectin-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2,
    -3, -4, -7, -8, or -9/Ecalectin is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 561603
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Galectin-10
    Met1-Arg142
    Accession # Q05315
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Galectin‑10 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes were stained with Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B) and Human IL‑2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC0041F and IC003A, respectively).
Detection of Human Galectin‑10 by Western Blot. Western blot shows lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Galectin‑10 in HL‑60 Human Cell Line. Galectin‑10 was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-10

Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors, and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one galectin domain (aa 6-138) that contains two dimerization motifs (aa 6-10 and 131-135). Two molecular weight isoforms of 15 and 14 kDa have been described. Human Galectin-10 has no known structural counterpart in rodents.    

  • Entrez Gene IDs:
    1178 (Human)
  • Alternate Names:
    Charcot-Leyden crystal proteinLGALS10A; CLC; EC 3.1.1.5; eosinophil lysophospholipase; GAL10; Gal-10; galectin 10; Galectin10; Galectin-10; LGALS10; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659
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