Human Galectin-10 Antibody Summary
-3, -4, -7, -8, or -9/Ecalectin is observed.
Accession # Q05315
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Galectin‑10 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes were stained with Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B) and Human IL-2 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC0041Fand IC003A).
Detection of Human Galectin‑10 by Western Blot. Western blot shows lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑10 in HL‑60 Human Cell Line. Galectin-10 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors, and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one galectin domain (aa 6-138) that contains two dimerization motifs (aa 6-10 and 131-135). Two molecular weight isoforms of 15 and 14 kDa have been described. Human Galectin-10 has no known structural counterpart in rodents.
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