Human Galectin-10 Antibody

Catalog # Availability Size / Price Qty
Detection of Galectin‑10 in Human PBMCs by Flow Cytometry.
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Product Details
Supplemental Products

Human Galectin-10 Antibody Summary

Species Reactivity
Detects human Galectin-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2,
-3, -4, -7, -8, or -9/Ecalectin is observed.
Monoclonal Mouse IgG2B Clone # 561603
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Galectin-10
Accession # Q05315
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Galectin-10 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Galectin‑10 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes were stained with Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B) and Human IL-2 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC0041Fand IC003A).

Western Blot Detection of Human Galectin-10 antibody by Western Blot. View Larger

Detection of Human Galectin‑10 by Western Blot. Western blot shows lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Galectin-10 antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC). View Larger

Galectin‑10 in HL‑60 Human Cell Line. Galectin-10 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-10

Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors, and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one galectin domain (aa 6-138) that contains two dimerization motifs (aa 6-10 and 131-135). Two molecular weight isoforms of 15 and 14 kDa have been described. Human Galectin-10 has no known structural counterpart in rodents.    

Entrez Gene IDs
1178 (Human)
Alternate Names
Charcot-Leyden crystal proteinLGALS10A; CLC; EC; eosinophil lysophospholipase; GAL10; Gal-10; galectin 10; Galectin10; Galectin-10; LGALS10; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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