|Detection of Galectin‑10 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes were stained with Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B) and Human IL‑2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC0041F and IC003A, respectively).|
|Detection of Human Galectin‑10 by Western Blot. Western blot shows lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Galectin‑10 in HL‑60 Human Cell Line. Galectin‑10 was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors, and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one galectin domain (aa 6-138) that contains two dimerization motifs (aa 6-10 and 131-135). Two molecular weight isoforms of 15 and 14 kDa have been described. Human Galectin-10 has no known structural counterpart in rodents.