Human gp130 Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human gp130 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat gp130, recombinant mouse gp130, recombinant human (rh) IL-1 sRI, rhIL-1 sRII, rhIL-2 sR beta, rhIL-2 sR gamma, rhIL‑4 sR, and rhIL-6 sR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130
    Leu24-Glu619 (Glu619Asp)
    Accession # P40189
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.70 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human gp130 (Catalog # 228-GP)
  • Neutralization
    Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 2‑10 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA). The ND50 is typically 2‑10 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

  • References:
    1. Narazaki, M. et al. (1993) Blood 82:1120.
    2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
  • Long Name:
    Glycoprotein 130
  • Entrez Gene IDs:
    3572 (Human); 16195 (Mouse); 25205 (Rat)
  • Alternate Names:
    CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. NF-kappaB-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma.
    Authors: McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, Benveniste E, Nozell S
    PLoS ONE, 2013;8(11):e78728.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer.
    Authors: Zhang Z, Lee JC
    Nat. Genet., 2012;44(8):852-860.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
    Authors: Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
    Fertil. Steril., 2009;91(6):2602-10.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
    Authors: Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O
    Fertil. Steril., 2006;86(4):1193-209.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression.
    Authors: Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL
    Cancer Res., 2005;65(19):8896-904.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy.
    Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H
    Circulation, 2003;107(6):798-802.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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