Human gp130 Antibody

Catalog # Availability Size / Price Qty
MAB628-SP
MAB628-500
MAB628-100
gp130 in Human Pancreas Tissue.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (4)

Human gp130 Antibody Summary

Species Reactivity
Human
Specificity
Detects human gp130 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) CNTF, rhIL-6, rhIL-6 R, recombinant mouse (rm) IL-6, rhIL-11, rhLIF, rhLIF R, rmLIF, or rhOSM is observed. This antibody blocks the human gp130-mediated bioactivities induced by IL-6, IL-11, LIF, OSM, and CNTF.
Source
Monoclonal Mouse IgG1 Clone # 28105
Purification
Protein A or G purified from ascites
Immunogen
Sf 21-derived recombinant human gp130 extracellular domain
Leu24-Glu619 (Glu619-Asp, predicted)
Accession # P40189
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Human gp130 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human gp130 Biotinylated Antibody (Catalog # BAF228)

Standard: Recombinant Human gp130 Protein (Catalog # 228-GP)

Neutralization
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry gp130 antibody in Human Pancreas Tissue by Immunohistochemistry (IHC-P). View Larger

gp130 in Human Pancreas Tissue. gp130 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. View Larger

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628). The ND50 is typically 0.02-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

gp130, also known as CD130, belongs to the class I cytokine receptor family. It functions as a signal transducing receptor component in the receptor complexes of the IL-6 family of cytokines including IL-6, IL-11, LIF, OSM, CNTF and CT-1.

Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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Citations for Human gp130 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Molecular Dissection of Pro-Fibrotic IL11 Signaling in Cardiac and Pulmonary Fibroblasts
    Authors: Anissa A. Widjaja, Sivakumar Viswanathan, Dong Jinrui, Brijesh K. Singh, Jessie Tan, Joyce Goh Wei Ting et al.
    Frontiers in Molecular Biosciences
  2. The pro-regenerative effects of hyperIL6 in drug-induced liver injury are unexpectedly due to competitive inhibition of IL11 signaling
    Authors: Jinrui Dong, Sivakumar Viswanathan, Eleonora Adami, Sebastian Schafer, Fathima F Kuthubudeen, Anissa A Widjaja et al.
    eLife
  3. Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality
    Authors: Ludovic Zimmerlin, Tea Soon Park, Jeffrey S. Huo, Karan Verma, Sarshan R. Pather, C. Conover Talbot et al.
    Development
  4. A myeloid–stromal niche and gp130 rescue in NOD2-driven Crohn’s disease
    Authors: Shikha Nayar, Joshua K. Morrison, Mamta Giri, Kyle Gettler, Ling-shiang Chuang, Laura A. Walker et al.
    Nature
  5. Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
    Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
    Mol. Hum. Reprod., 2004-03-25;10(5):313-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Structural basis of activation and antagonism of receptor signaling mediated by interleukin-27
    Authors: Katarzyna Składanowska, Yehudi Bloch, Jamie Strand, Kerry F. White, Jing Hua, Daniel Aldridge et al.
    Cell Reports

FAQs

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Reviews for Human gp130 Antibody

Average Rating: 4 (Based on 4 Reviews)

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Human gp130 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human gp130 Antibody
By Anonymous on 01/09/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human gp130 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse

Human gp130 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human