Human Growth Hormone R/GHR Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Growth Hormone R/GHR in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse GHR and recombinant rat GHR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Growth Hormone R/GHR
    Ala27-Tyr264
    Accession # P10912
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Growth Hormone R/GHR Fc Chimera (Catalog # 1210-GR)
  • Flow Cytometry
    0.25 µg/106 cells
    Human whole blood CD19+ B cells
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human breast cancer tissue
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize Growth Hormone R/GHR-mediated inhibition of proliferation in the Nb2‑11 rat lymphoma cell line. The Neutralization Dose (ND50) is typically 0.125-0.5 µg/mL in the presence of 10 ng/mL Recombinant Human Growth Hormone R/GHR Fc Chimera and 0.2 ng/mL Recombinant Human Growth Hormone.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Human Growth Hormone R/GHR Antibody. Recombinant Human Growth Hormone R/GHR Fc Chimera (Catalog #
1210‑GR) inhibits Recombinant Human Growth Hormone (Catalog # 1067‑GH) induced proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human Growth Hormone (0.2 ng/mL) activity elicited by Recombinant Human Growth Hormone R/GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1210). The ND50 is typically
0.125‑0.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).

  • References:
    1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
    2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
    3. Clark, R. (1997) Endocr. Rev. 18:157.
    4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
    5. Leung, D.W. et al. (1987) Nature 330:537.
    6. Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394.
    7. Amit, T. et al. (1997) Endocr. Metab. 82:3813.
    8. Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265.
    9. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
    10. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.
  • Long Name:
    Growth Hormone Receptor
  • Entrez Gene IDs:
    2690 (Human); 14600 (Mouse); 25235 (Rat)
  • Alternate Names:
    GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains.
    Authors: Zhang Z, Hatano H, Shaw J, Olde Nordkamp M, Jiang G, Li D, Kollnberger S
    PLoS ONE, 2015;10(6):e0129063.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Growth hormone promotes lymphangiogenesis.
    Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
    Am. J. Pathol., 2008;173(2):586-97.
    Species: Human
    Sample Type: Cell Lysates
    Application: IHC Frozen
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