Human Growth Hormone R/GHR Antibody Summary
Accession # P10912
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Human Growth Hormone R/GHR Antibody. Recombinant Human Growth Hormone R/GHR Fc Chimera (Catalog # 1210-GR) inhibits Recombinant Human Growth Hormone (Catalog # 1067-GH) induced proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human Growth Hormone (0.2 ng/mL) activity elicited by Recombinant Human Growth Hormone R/GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1210). The ND50 is typically 0.125-0.5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone R/GHR
Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).
- Goffin, V. et al. (1996) Endocrine Rev. 17:385.
- Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
- Clark, R. (1997) Endocr. Rev. 18:157.
- Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
- Leung, D.W. et al. (1987) Nature 330:537.
- Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394.
- Amit, T. et al. (1997) Endocr. Metab. 82:3813.
- Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265.
- Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
- Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.
Citations for Human Growth Hormone R/GHR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains.
Authors: Zhang Z, Hatano H, Shaw J, Olde Nordkamp M, Jiang G, Li D, Kollnberger S
PLoS ONE, 2015;10(6):e0129063.
Sample Types: Whole Cells
Applications: Flow Cytometry
Growth hormone promotes lymphangiogenesis.
Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
Am. J. Pathol., 2008;173(2):586-97.
Sample Types: Whole Cells
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