Human HGF R/c-MET Antibody AF276: R&D Systems

Human HGF R/c-MET Antibody

(14 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse HGF R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
    Glu25-Thr932
    Accession # P08581
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human HGF R/c-MET Fc Chimera (Catalog # 358-MT)
  • Flow Cytometry
    2.5 µg/106 cells
    MDA‑MB‑231 human breast cancer cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF (Catalog # 294-HGN) to immobilized Recombinant Human HGF R/c-MET Fc Chimera (Catalog # 358‑MT) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
HGF R/c‑MET in HT‑29 and U937 Human Cell Line. HGF R/c‑MET was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (left panel) and U937 human histiocytic lymphoma cell line (right panel) using Goat Anti-Human HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
HGF R/c‑MET in Human Liver. HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
HGF R/c‑MET in Human Skin. HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF R/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% aa sequence identity with canine, mouse, and rat HGF R.

  • References:
    1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
    2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
    3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
    4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
    5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
    6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
    7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
    8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
    9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
    10. Ponzetto, C. et al. (1994) Cell 77:261.
    11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
    12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
    13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
    14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
    15. Wang, X. et al. (2002) Mol. Cell 9:411.
    16. Trusolino, L. et al. (2001) Cell 107:643.
    17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
    18. Conrotto, P. et al. (2004) Oncogene 23:5131.
    19. Follenzi, A. et al. (2000) Oncogene 19:3041.
    20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
  • Long Name:
    Hepatocyte Growth Factor Receptor
  • Entrez Gene IDs:
    4233 (Human); 17295 (Mouse)
  • Alternate Names:
    AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
    Authors: Rachel Barrow-McG
    Nat Commun, 2016;7(0):11942.
  2. Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer
    Authors: Deborah R Kaye
    PLoS ONE, 2016;11(6):e0157130.
    Species: Human
    Sample Type: Plasma
    Application: ELISA detection
  3. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
    Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
    Nat Commun, 2015;6(0):6776.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
    Authors: Tabernero J, Elez M, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras M, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J
    Clin Cancer Res, 2014;20(10):2793-804.
    Species: Human
    Sample Type: Serum
    Application: ELISA detection
  5. Mutant p53 enhances MET trafficking and signalling to drive cell scattering and invasion.
    Authors: Muller, P A J, Trinidad, A G, Timpson, P, Morton, J P, Zanivan, S, van den Berghe, P V E, Nixon, C, Karim, S A, Caswell, P T, Noll, J E, Coffill, C R, Lane, D P, Sansom, O J, Neilsen, P M, Norman, J C, Vousden, K H
    Oncogene, 2013;32(10):1252-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins.
    Authors: Hasenauer S, Malinger D, Koschut D, Pace G, Matzke A, von Au A, Orian-Rousseau V
    PLoS ONE, 2013;8(4):e62357.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Identification of a pivotal endocytosis motif in c-Met and selective modulation of HGF-dependent aggressiveness of cancer using the 16-mer endocytic peptide.
    Authors: Cho K, Park J, Park C, Lee D, Lee E, Kim D, Kim K, Yoon S, Park Y, Kim E, Cho S, Jang S, Park B, Chi S, Yoo S, Jang M, Kim H, Kim E, Jo K, Park Y
    Oncogene, 2012;32(8):1018-29.
    Species: Human
    Sample Type:
  8. c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
    Authors: Szabo R, Rasmussen AL, Moyer AB
    Oncogene, 2011;30(17):2003-16.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.
    Authors: Abella JV, Parachoniak CA, Sangwan V, Park M
    J. Biol. Chem., 2010;285(32):24956-67.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells.
    Authors: Xu L, Nilsson MB, Saintigny P, Cascone T, Herynk MH, Du Z, Nikolinakos PG, Yang Y, Prudkin L, Liu D, Lee JJ, Johnson FM, Wong KK, Girard L, Gazdar AF, Minna JD, Kurie JM, Wistuba II, Heymach JV
    Oncogene, 2010;29(18):2616-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Effect of human patient plasma ex vivo treatment on gene expression and progenitor cell activation of primary human liver cells in multi-compartment 3D perfusion bioreactors for extra-corporeal liver support.
    Authors: Schmelzer E, Mutig K, Schrade P, Bachmann S, Gerlach JC, Zeilinger K
    Biotechnol. Bioeng., 2009;103(4):817-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Decorin is a novel antagonistic ligand of the Met receptor.
    Authors: Goldoni S, Humphries A, Nystrom A, Sattar S, Owens RT, McQuillan DJ, Ireton K, Iozzo RV
    J. Cell Biol., 2009;185(4):743-54.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.
    Authors: Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM
    Arthritis Rheum., 2007;56(7):2432-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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