Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1069511
View all HLA Class I Primary Antibodies »

Product Specifications

Immunogen

Membranes from human tonsillar lymphocytes

Specificity

Detects the human HLA-A protein by binding to its peptide region which includes Aspartic Acid (ASP) 338 as determined by ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human HLA Class I Antibody

Detection of Human HLA Class I by Western Blot.

Western Blot shows lysates of MDA‑MB‑231 human breast cancer cell line, Raji human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB11502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA Class I at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of HLA Class I in Human Spleen.

HLA Class I was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB11502) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of HLA Class I in PBMC by Flow Cytometry

PBMC were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB11502) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human HLA Class I Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Detection of HLA Class I in PBMC by Flow Cytometry

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

2 µg/mL
Sample: MDA-MB-231 human breast cancer cell line, Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HLA Class I

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

References

  1. Barnstable, C.J. et al. (1978) Cell 14:9.

Long Name

Class I Human Leukocyte Antigen

Alternate Names

HLA-A, HLA-ABC

Entrez Gene IDs

3105 (Human)

Gene Symbol

HLA-A

Additional HLA Class I Products

Product Documents for Human HLA Class I Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HLA Class I Antibody

For research use only

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Protocols

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