Human IFN‑ beta Antibody

Name: Human IFN‑ beta Antibody
Brand: R&D Systems
SKU: MAB8144
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB8144

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Product Specifications
Scientific Data
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Preparation & Storage
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 944403

View all IFN-beta Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human IFN‑ beta
Met1-Asn187
Accession # P01574

Specificity

Detects human IFN‑ beta in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human IFN‑ beta Antibody

Human IFN‑ beta ELISA Standard Curve.

Recombinant Human IFN-beta protein was serially diluted 2-fold and captured by Mouse Anti-Human IFN-beta Monoclonal Antibody (Catalog # MAB8144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IFN-beta Monoclonal Antibody(Catalog # MAB8143) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IFN‑ beta Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IFN‑ beta Monoclonal Antibody (Catalog # MAB8143).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IFN-beta DuoSet ELISA Kit (Catalog # DY814-05) for convenient development of a sandwich ELISA or the Human IFN-beta Quantikine ELISA Kit (Catalog # DIFNB0) for a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.