Human IGFBP-3 Antibody Summary
Accession # CAA46087
Human IGFBP-3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IGFBP‑3 in Human Placenta. IGFBP-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human IGFBP-3 Monoclonal Antibody (Catalog # MAB305) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Human IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked and two potential O-linked glycosylation sites. Human IGFBP-3 is expressed in multiple tissues. The highest expression level is found in the non-paranchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty. Human IGFBP-3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).
- Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
- Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
- Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1.
Citations for Human IGFBP-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 7
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Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer
Authors: HJ Shih, HF Chang, CL Chen, PL Torng
Scientific Reports, 2021-11-25;11(1):22925.
Species: Human, Xenograft
Sample Types: Recombinant Proteins, Whole Cells, Whole Tissue
Applications: ICC, IHC, Neutralization
Enhanced Expression of IGFBP-3 Reduces Radiosensitivity and Is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma
Authors: J Sakata, A Hirosue, R Yoshida, Y Matsuoka, K Kawahara, H Arita, H Nakashima, T Yamamoto, M Nagata, S Kawaguchi, S Gohara, Y Nagao, K Yamana, R Toya, R Murakami, Y Kuwahara, M Fukumoto, H Nakayama
Cancers (Basel), 2020-02-20;12(2):.
Sample Types: Whole Tissue
The participation of insulin-like growth factor-binding protein 3 released by astrocytes in the pathology of Alzheimer's disease.
Authors: Watanabe K, Uemura K, Asada M, Maesako M, Akiyama H, Shimohama S, Takahashi R, Kinoshita A
Mol Brain, 2015-12-04;8(1):82.
Sample Types: Tissue Homogenates
Applications: Western Blot
IGFBP3 promotes esophageal cancer growth by suppressing oxidative stress in hypoxic tumor microenvironment.
Authors: Natsuizaka M, Kinugasa H, Kagawa S, Whelan K, Naganuma S, Subramanian H, Chang S, Nakagawa K, Rustgi N, Kita Y, Natsugoe S, Basu D, Gimotty P, Klein-Szanto A, Diehl J, Nakagawa H
Am J Cancer Res, 2014-01-15;4(1):29-41.
Sample Types: Whole Tissue
Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
Authors: Elzi, David J, Lai, Yanlai, Song, Meihua, Hakala, Kevin, Weintraub, Susan T, Shiio, Yuzuru
Proc Natl Acad Sci U S A, 2012-07-09;109(30):12052-7.
Sample Types: Whole Tissue
Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
Authors: Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot
Epidermal growth factor receptor regulates aberrant expression of insulin-like growth factor-binding protein 3.
Authors: Takaoka M, Harada H, Andl CD, Oyama K, Naomoto Y, Dempsey KL, Klein-Szanto AJ, El-Deiry WS, Grimberg A, Nakagawa H
Cancer Res., 2004-11-01;64(21):7711-23.
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P
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