Human IGFBP-3 Antibody MAB305-100: R&D Systems

Human IGFBP-3 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IGFBP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) IGF‑I R, rhIGFBP-1, rhIGFBP-2, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 84728
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IGFBP‑3
    Gly28-Lys291
    Accession # CAA46087
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IGFBP‑3 (Catalog # 675-B3)
  • Immunohistochemistry
    8-25 µg/mL
    See below
    • Human IGFBP-3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IGFBP‑3 Antibody (Catalog # MAB305)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IGFBP‑3 Biotinylated Antibody (Catalog # BAF675)
  • ELISA Standard
     
    Recombinant Human IGFBP-3 Protein, CF (Catalog # 675-B3)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
IGFBP‑3 in Human Placenta. IGFBP‑3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked and two potential O-linked glycosylation sites. Human IGFBP-3 is expressed in multiple tissues. The highest expression level is found in the non-paranchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty. Human IGFBP-3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).

  • References:
    1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
    2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
    3. Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1.
  • Long Name:
    Insulin-like Growth Factor Binding Protein 3
  • Entrez Gene IDs:
    3486 (Human); 16009 (Mouse)
  • Alternate Names:
    acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. The participation of insulin-like growth factor-binding protein 3 released by astrocytes in the pathology of Alzheimer's disease.
    Authors: Watanabe K, Uemura K, Asada M, Maesako M, Akiyama H, Shimohama S, Takahashi R, Kinoshita A
    Mol Brain, 2015;8(1):82.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
    Authors: Elzi, David J, Lai, Yanlai, Song, Meihua, Hakala, Kevin, Weintraub, Susan T, Shiio, Yuzuru
    Proc Natl Acad Sci U S A, 2012;109(30):12052-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
    Authors: Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
    Endocrinology, 2011;152(6):2184-96.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Epidermal growth factor receptor regulates aberrant expression of insulin-like growth factor-binding protein 3.
    Authors: Takaoka M, Harada H, Andl CD, Oyama K, Naomoto Y, Dempsey KL, Klein-Szanto AJ, El-Deiry WS, Grimberg A, Nakagawa H
    Cancer Res., 2004;64(21):7711-23.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Isotype Controls
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