Human IkB-epsilon Antibody

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Detection of Human IkB-epsilon  by Western Blot.
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Product Details
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Human IkB-epsilon Antibody Summary

Species Reactivity
Detects endogenous human and mouse IkB-epsilon in Western blots.
Monoclonal Mouse IgG2B Clone # 418515
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IkB-epsilon
Accession # Q96F31
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IkB-e antibody by Western Blot. View Larger

Detection of Human IkB-epsilon by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line, PC-3 human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human IkB-e Monoclonal Antibody (Catalog # MAB4300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-e at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name
Entrez Gene IDs
4794 (Human); 18037 (Mouse)
Alternate Names
epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

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