|Detection of Human IkB-epsilon by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line, PC‑3 human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human IkB-epsilon Monoclonal Antibody (Catalog # MAB4300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-epsilon at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.|
NF kappa B inhibitor epsilon (IkB epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided