Human IL-10 Antibody

Catalog # Availability Size / Price Qty
MAB2171-SP
MAB2171-100
MAB2171-500
Detection  of Human IL‑10 by Western Blot.
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Product Details
Citations (11)
FAQs
Supplemental Products
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Human IL-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.
Source
Monoclonal Mouse IgG2B Clone # 25209
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.005-0.03 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human IL‑10 by Western Blot. View Larger

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody.

Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171). The ND50 is typically 0.005‑0.03 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Citations for Human IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
    Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
    Arthritis Res. Ther., 2017;19(1):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. Oral fibroblasts modulate the macrophage response to bacterial challenge
    Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
    Sci Rep, 2017;7(1):11516.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma.
    Authors: Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F
    PLoS ONE, 2014;9(6):e100909.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  4. HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.
    Authors: Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E
    J Virol, 2014;88(12):6672-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
    Authors: Svajger U, Obermajer N, Jeras M
    J Leukoc Biol, 2014;95(1):33-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  6. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  7. DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function.
    Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M
    J. Leukoc. Biol., 2011;89(6):893-905.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  8. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
    Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM
    J. Immunol., 2009;182(5):2929-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  9. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  10. Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
    Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
    J. Immunol., 2007;179(4):2359-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  11. Effect of IL-21 on NK cells derived from different umbilical cord blood populations.
    Authors: Perez S, Mahaira L, Sotiropoulou P, Gritzapis A, Iliopoulou E, Niarchos D, Cacoullos N, Kavalakis Y, Antsaklis A, Sotiriadou N, Baxevanis C, Papamichail M
    Int Immunol, 0;18(1):49-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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