Human IL-15R alpha Antibody

R&D Systems | Catalog # AF247

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Capture, ELISA Development, CyTOF-reported

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-15R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human
IL‑15 R alpha
Ile31-Thr172
Accession # EAW86418

Specificity

Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant humanIL‑2 R gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-15R alpha Antibody

IL-15 Ra antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC).

IL‑15 R alpha in HDLM‑2 Human Cell Line.

IL-15 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL‑15 Ra antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

IL‑15 R alpha in Human Prostate Cancer Tissue.

IL‑15 Ra was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody.

IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody.

Recombinant Human IL-15 Ra Fc Chimera (Catalog # 147-IR) inhibits Recombinant Human IL-15 (Catalog # 247-IL) induced proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-15 (2 ng/mL) activity elicited by Recombinant Human IL-15 Ra Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND50 is typically 1-4 µg/mL.
Detection of Human IL-15R alpha by ELISA

Detection of Human IL-15R alpha by ELISA

Measured levels of sIL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluids from RA and OA patients. Cytokine concentration of IL-15Ralpha (A), IL-15 (B), TNFalpha (C) and IL-6 (D) were measured in in duplicate SF samples from RA (n = 30) and OA (n = 30) by ELISA. Box plot represents media ± SD. Differences between two groups were performed by Mann–Whitney U test for nonparametric data. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25879761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-15R alpha in Human Pancreas.

Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for IL15Ra mRNA (ACD RNAScope Probe, catalog #532368; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human IL15Ra polyclonal antibody (R&D Systems catalog # AF247) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands.

Detection of IL-15R alpha in human monocytes treated with LPS(100ng/mL) and IFNg(10ng/mL) for 24h by Flow Cytometry

Human monocytes treated with LPS(100ng/mL) and IFNg(10ng/mL) for 24h (filled histogram) or resting human monocytes (open histogram) were stained with Goat Anti-Human IL-15R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-15R alpha Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Dual RNAscope ISH-IHC Compatible

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Flow Cytometry

0.25 µg/106 cells
Sample: Human monocytes treated with LPS and IFNg 

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed human Hodgkin's lymphoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑15 R alpha Fc Chimera (Catalog # 147-IR)

Neutralization

Measured by its ability to neutralize IL‑15 R alpha -mediated inhibition of proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑15 R alpha Fc Chimera and 2 ng/mL Recombinant Human IL‑15.

Reviewed Applications

Read 2 reviews rated 4 using AF247 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.
IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of
IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References

  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

EAW86418

Additional IL-15R alpha Products

Product Documents for Human IL-15R alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-15R alpha Antibody

For research use only

Citations for Human IL-15R alpha Antibody

Customer Reviews for Human IL-15R alpha Antibody (2)

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  • Human IL-15R alpha Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with mAb and protein standard (147-IR-100) to quantify IL-15R alpha in human serum and plasma. This combination could not detect IL-15R alpha in our samples but generated a good standard curve.
    Human IL-15R alpha Antibody AF247
  • Human IL-15R alpha Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 05/01/2020
    Ab was used as capture antibody in human serum ELISA
    Human IL-15R alpha Antibody AF247

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