Human IL-17F Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL‑17A, B, C, D, or E.
  • Source
    Monoclonal Mouse IgG2B Clone # 197301
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-17F
    Arg31-Gln163
    Accession # Q96PD4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑17F in Activated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13351) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunocytochemistry
IL‑17F in Human Th17 Cells. IL‑17F was detected in immersion fixed human T helper 17 cells using Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13351) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17F
Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions.
  • Long Name:
    Interleukin 17F
  • Entrez Gene IDs:
    112744 (Human); 257630 (Mouse); 301291 (Rat)
  • Alternate Names:
    Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
    Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
    J Allergy Clin Immunol, 2013;131(4):1117-29, 1129.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  2. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Isotype Controls
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Mouse IgG2B Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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