Human IL-1ra/IL-1F3 Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑1ra/IL-1F3 in ELISAs. In ELISAs, this antibody shows less than 0.2% cross-reactivity with recombinant mouse (rm) IL‑1ra and no cross-reactivity with rhIL‑1 alpha, rpIL‑1 alpha, rmIL‑1 alpha, rrIL‑1 alpha, rhIL‑1 beta, rpIL‑1 beta, rmIL‑1 beta, rrIL‑1 beta, rhIL‑1 RI, or rhIL‑1 RII.
  • Source
    Monoclonal Mouse IgG2A Clone # 10309
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IL‑1ra/IL-1F3
    Arg26-Glu177
    Accession # P18510
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human IL-1ra/IL-1F3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IL‑1ra/IL‑1F3 Antibody (Catalog # MAB280)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IL‑1ra/IL‑1F3 Biotinylated Antibody (Catalog # BAF280)
  • ELISA Standard
     
    Recombinant Human IL-1ra/IL-1F3 Protein (Catalog # 280-RA)
  • Neutralization
    Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 50 ng/mL Recombinant Human IL‑1ra/IL‑1F3, 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody. Recombinant Human IL‑1ra/IL‑1F3 (Catalog # 280-RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 alpha /IL‑1F1 (50 pg/mL) activity elicited by Recombinant Human IL‑1ra/IL‑1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280). The ND50 is typically 10-20 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

  • Long Name:
    Interleukin 1 Receptor Antagonist
  • Entrez Gene IDs:
    3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
  • Alternate Names:
    DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Interleukin-36 potently stimulates human M2 macrophages, Langerhans cells and keratinocytes to produce pro-inflammatory cytokines
    Authors: Damien Dietrich
    Cytokine, 2016;84(0):88-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  3. Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
    Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C
    Cytokine, 2005;31(2):153-60.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  5. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Array Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Siteclick antibody biotinylation.

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 Siteclick antibody biotinylation Mouse Anonymous 10/27/2017
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Summary

ApplicationSiteclick antibody biotinylation
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse

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