IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Arg26-Glu177
Accession # P18510
Specificity
Clonality
Host
Isotype
Endotoxin Level
Scientific Data Images for Human IL‑1ra/IL‑1F3 Antibody
IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody.
Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND50 is typically 10-20 µg/mL.
Applications for Human IL‑1ra/IL‑1F3 Antibody
Neutralization
Human IL-1ra/IL-1F3 Sandwich Immunoassay
Reviewed Applications
Read 3 reviews rated 4.7 using MAB280 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-1ra/IL-1F3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-1ra/IL-1F3 Products
Product Documents for Human IL‑1ra/IL‑1F3 Antibody
Product Specific Notices for Human IL‑1ra/IL‑1F3 Antibody
For research use only
Citations for Human IL‑1ra/IL‑1F3 Antibody
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Customer Reviews for Human IL‑1ra/IL‑1F3 Antibody (3)
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Customer Images
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 11/18/2019
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Application: Simple WesternSample Tested: B16-F1 mouse melanoma cell lineSpecies: MouseVerified Customer | Posted 04/05/2018
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Application: Siteclick antibody biotinylationSample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell lineSpecies: MouseVerified Customer | Posted 10/27/2017
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