Human IL-1ra/IL-1F3 Antibody

Catalog # Availability Size / Price Qty
AF-280-NA
AF-280-SP
Detection of Human IL‑1ra/IL‑1F3 by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)

Human IL-1ra/IL-1F3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 15% cross-reactivity with recombinant mouse IL-1ra is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑1ra/IL-1F3
Arg26-Glu177
Accession # P18510
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically ≤4 µg/mL in the presence of 50 ng/mL Recombinant Human IL‑1ra/IL‑1F3 and 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-1ra/IL-1F3 antibody by Western Blot. View Larger

Detection of Human IL‑1ra/IL‑1F3 by Western Blot. Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL-1ra/IL-1F3 antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

IL‑1ra/IL‑1F3 in Human Skin. IL-1ra/IL-1F3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse IL-1ra/IL-1F3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse IL‑1ra/IL‑1F3 by Simple WesternTM. Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, A431 human epithelial carcinoma cell line, human skin cancer tissue, and mouse skin cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for IL-1ra/IL-1F3 at approximately 24-26 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody. View Larger

IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody. Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND50 is typically ≤ 4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name
Interleukin 1 Receptor Antagonist
Entrez Gene IDs
3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Alternate Names
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

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Citations for Human IL-1ra/IL-1F3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Interleukin 1&beta/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy
    Authors: Y Chen, Z Yang, B Deng, D Wu, Y Quan, Z Min
    Oncol. Rep., 2020-01-22;43(3):908-918.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Prostaglandin E2 Indicates Therapeutic Efficacy Of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury
    Authors: DJ Kota, KS Prabhakara, N Toledano-F, D Bhattarai, Q Chen, B DiCarlo, P Smith, F Triolo, PL Wenzel, CS Cox, SD Olson
    Stem Cells, 2017-03-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model
    Authors: JH Lee, EH Kam, JM Kim, SY Kim, EJ Kim, SY Cheon, BN Koo
    Biomol Ther (Seoul), 2017-03-01;25(2):149-157.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation.
    Authors: Rider P, Carmi Y, Yossef R, Guttman O, Eini H, Azam T, Dinarello C, Lewis E
    J Immunol, 2015-07-08;195(4):1705-12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.
    Authors: Megjugorac NJ, Gallagher GE, Gallagher G
    Blood, 2010-03-16;115(21):4185-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007-03-12;151(1):115-27.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
    Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
    Arthritis Rheum., 2007-02-01;56(2):674-87.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  9. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004-08-01;21(2):241-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
    Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
    J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human IL-1ra/IL-1F3 Antibody

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Human IL-1ra/IL-1F3 Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Primate

Used as a capture antibody in our NHP ELISA


Human IL-1ra/IL-1F3 Antibody
By Anonymous on 11/18/2019
Application: ELISA Sample Tested: Serum Species: Human