Human LIF Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LIF in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhOSM, rhTGF-beta 1, rhTNF-alpha, rhTNF-beta, recombinant mouse (rm) IL-1 beta, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmGM-CSF, bovine (b) FGF acidic, bFGF basic, human (h) PDGF, porcine (p) PDGF, hTGF-beta 1, pTGF-beta 1.2, or pTGF-beta 2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 9824
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human LIF
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human lung
    • Human LIF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human LIF Antibody (Catalog # MAB250)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human LIF Biotinylated Antibody (Catalog # BAF250)
  • Neutralization
    Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.06-0.2 µg/mL in the presence of 1.5 ng/mL Recombinant Human LIF. Human LIF Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) is recommended for neutralization.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB250). The ND50 is typically 0.06-0.2 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF
LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells (17).
  • References:
    1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
    2. Gough, N.M. (1992) Growth Factors 7:175.
    3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
    4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
    5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
    6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
    7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
    8. Williams, R.L. et al. (1988) Nature 336:684.
    9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
    10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
    11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
    12. Davis, S. et al. (1993) Science 260:18054.
    13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
    14. Gearing, D.P. et al. (1991) Science 255:1434.
    15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
    16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
  • Long Name:
    Leukemia Inhibitory Factor
  • Entrez Gene IDs:
    3976 (Human); 16878 (Mouse); 403449 (Canine)
  • Alternate Names:
    CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
    Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
    J. Biol. Chem., 2011;286(39):34271-85.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  2. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  3. Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
    Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
    Mol. Hum. Reprod., 2004;10(5):313-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  4. The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
    Authors: Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
    Arthritis Rheum., 2001;44(10):2392-401.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
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