Human LMP2/PSMB9 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LMP2/PSMB9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human PSMB6 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 792520
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human LMP2/PSMB9
    Thr21-Glu219
    Accession # P28065
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human LMP2/PSMB9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line treated (+) with 100 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMP2/PSMB9 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
LMP2/PSMB9 in HeLa Human Cell Line. LMP2/PSMB9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cells stimulated with Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours using Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LMP2/PSMB9
LMP2 (large multifunctional peptidase 2), also called PSMB9 (proteasome subunit beta 9) or PSMB6i, is a 22 kDa broad-specificity aminopeptidase of the T1B family that is a component of the 20S immunoproteasome core (a7b7b7a7) of the 26S proteasome particle. Induction of LMP2 by IFN-g, IRF-1, TNF-a, CD40L, or heat shock in immune-responsive cells or cancer cells promotes replacement of proteasome subunit PSMB6 by LMP2 and processing of peptides for presentation by MHC I. After auto-cleavage between amino acids (aa) 20-21, human LMP2 (aa 21-219) shares 91% aa sequence identity with mouse and rat LMP2. Alternate splicing creates a short form, LMP2.S, which lacks aa 4-13 within the propeptide.
  • Long Name:
    Proteasome (Prosome, Macropain) Subunit, beta Type, 9
  • Entrez Gene IDs:
    5698 (Human); 16912 (Mouse); 24967 (Rat)
  • Alternate Names:
    beta1i; LMP2; LMP2MGC70470; Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalpeptidase 2); proteasome catalytic subunit 1i; Proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta type-9; Proteasome subunit beta-1i; proteasome-related gene 2; PSMB6iproteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalprotease 2); PSMB9; Really interesting new gene 12 protein; RING12; RING12EC 3.4.25.1
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