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Human LMP2/PSMB9 Antibody

Catalog #: MAB7709 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7709
MAB7709-SP

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Detection of Human LMP2/PSMB9 by Western Blot.
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Human LMP2/PSMB9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LMP2/PSMB9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human PSMB6 is observed.
Source
Monoclonal Mouse IgG2B Clone # 792520
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LMP2/PSMB9
Thr21-Glu219
Accession # P28065
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LMP2/PSMB9 antibody by Western Blot. View Larger

Detection of Human LMP2/PSMB9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line treated (+) with 100 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMP2/PSMB9 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry LMP2/PSMB9 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

LMP2/PSMB9 in HeLa Human Cell Line. LMP2/PSMB9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cells stimulated with Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours using Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody (Catalog # MAB7709) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMP2/PSMB9

LMP2 (large multifunctional peptidase 2), also called PSMB9 (proteasome subunit beta 9) or PSMB6i, is a 22 kDa broad-specificity aminopeptidase of the T1B family that is a component of the 20S immunoproteasome core (a7b7b7a7) of the 26S proteasome particle. Induction of LMP2 by IFN-g, IRF-1, TNF-a, CD40L, or heat shock in immune-responsive cells or cancer cells promotes replacement of proteasome subunit PSMB6 by LMP2 and processing of peptides for presentation by MHC I. After auto-cleavage between amino acids (aa) 20-21, human LMP2 (aa 21-219) shares 91% aa sequence identity with mouse and rat LMP2. Alternate splicing creates a short form, LMP2.S, which lacks aa 4-13 within the propeptide.

Long Name
Proteasome (Prosome, Macropain) Subunit, beta Type, 9
Entrez Gene IDs
5698 (Human); 16912 (Mouse); 24967 (Rat)
Alternate Names
beta1i; LMP2; LMP2MGC70470; Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalpeptidase 2); proteasome catalytic subunit 1i; Proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta type-9; Proteasome subunit beta-1i; proteasome-related gene 2; PSMB6iproteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalprotease 2); PSMB9; Really interesting new gene 12 protein; RING12; RING12EC 3.4.25.1

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