Human LRP-6 Antibody

  (2 citations)     
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Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects human LRP-6 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) LRP-6 and no cross-reactivity with recombinant human (rh) LRP-1, -4, -5, rhLRP-6 (intracellular domain), or rhLDL R is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 255302
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LRP-6
    Ala20-Pro1368
    Accession # O75581
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LRP-6 in MDA-
MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP‑6 Monoclonal Antibody (Catalog # MAB1505, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry
LRP‑6 in DU145 Human Cell Line. LRP‑6 was detected in immersion fixed DU145 human prostate carcinoma cell line using Mouse Anti-Human LRP‑6 Monoclonal Antibody (Catalog # MAB1505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-6
LRP-6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP-6 associates with select Fzd multipass receptors; in the PTH system, LRP-6 complexes with PTH1R. Mature human LRP-6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs.
  • Long Name:
    LDL Receptor-related Protein 6
  • Entrez Gene IDs:
    4040 (Human); 16974 (Mouse)
  • Alternate Names:
    ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds
    Proc Natl Acad Sci USA, 2016;113(21):E2945-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
    Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
    Nature, 2012;485(7397):195-200.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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