Human LRP-6 Antibody

Catalog # Availability Size / Price Qty
MAB1505
MAB1505-SP
Detection of LRP-6 in MDA- MB-231 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
FAQs
Supplemental Products
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Human LRP-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRP-6 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) LRP-6 and no cross-reactivity with recombinant human (rh) LRP-1, -4, -5, rhLRP-6 (intracellular domain), or rhLDL R is observed.
Source
Monoclonal Mouse IgG2A Clone # 255302
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRP-6
Ala20-Pro1368
Accession # O75581
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of LRP-6 antibody in MDA- MB-231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LRP-6 in MDA- MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP-6 Monoclonal Antibody (Catalog # MAB1505, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry LRP-6 antibody in DU145 Human Cell Line by Immunocytochemistry (ICC). View Larger

LRP‑6 in DU145 Human Cell Line. LRP-6 was detected in immersion fixed DU145 human prostate carcinoma cell line using Mouse Anti-Human LRP-6 Monoclonal Antibody (Catalog # MAB1505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-6

LRP-6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP-6 associates with select Fzd multipass receptors; in the PTH system, LRP-6 complexes with PTH1R. Mature human LRP-6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs.

Long Name
LDL Receptor-related Protein 6
Entrez Gene IDs
4040 (Human); 16974 (Mouse)
Alternate Names
ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6

Product Datasheets

Citations for Human LRP-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds
    Proc Natl Acad Sci USA, 2016;113(21):E2945-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
    Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
    Nature, 2012;485(7397):195-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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