Detection of Human Mcl‑1 by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl‑1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mcl‑1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mcl-1 (myeloid cell leukemia-1; also known as Bcl-2-like protein 3) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms: 40 kDa Mcl-1L (long; 350 amino acids (aa)) enhances cell survival by inhibiting apoptosis, while 32 kDa Mcl-1S (short; 271 aa with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation of Mcl-1. Within the first 230 aa, human Mcl-1 shares ~68% aa identity with mouse and rat Mcl-1.
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