Human Mcl-1 Antibody Summary
Accession # Q07820
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Mcl‑1 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mcl-1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot Shows Human Mcl‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Mcl-1 knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mcl-1 at approximately 40 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mcl-1 (myeloid cell leukemia-1; also known as Bcl-2-like protein 3) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms: 40 kDa Mcl-1L (long; 350 amino acids (aa)) enhances cell survival by inhibiting apoptosis, while 32 kDa Mcl-1S (short; 271 aa with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation of Mcl-1. Within the first 230 aa, human Mcl-1 shares ~68% aa identity with mouse and rat Mcl-1.
Citation for Human Mcl-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions
Authors: M Mues, L Karra, D Romero-Moy, A Wandler, MJ Hangauer, O Ksionda, Y Thus, M Lindenberg, K Shannon, MT McManus, JP Roose
Cell Rep, 2019;27(2):631-647.e5.
Sample Types: Whole Cells
Applications: Flow Cytometry
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Antibody was also used in ELISA.