Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Dual RNAscope ISH-IHC Compatible, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑7
Leu18-Lys267
Accession # P09237

Specificity

Detects human MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MMP-7 is observed, and less than 1% cross-reactivity with recombinant human (rh) MMP‑8 and rhMMP-12 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP‑7 Antibody

Detection of Human MMP-7 antibody by Western Blot.

Detection of Human MMP‑7 by Western Blot.

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP-7 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

MMP‑7 in Human Pancreas.

MMP-7 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human MMP-7 antibody by Simple WesternTM.

Detection of Human MMP‑7 by Simple WesternTM.

Simple Western lane view shows lysates of normal stem cells (negative control) and Crohn's stem cells, loaded at 0.2 mg/mL. A specific band was detected for MMP-7 at approximately 10 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization of MMP‑7 Activity by Human MMP‑7 Antibody.

Neutralization of MMP‑7 Activity by Human MMP‑7 Antibody.

The cleavage of Mca-PLGL-Dpa-AR-NH2(10 µM, Catalog # ES001) by Recombinant Human MMP-7 (0.2 µg/mL, Catalog # 907-MP) is measured after preincubation with increasing concentrations of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907). The ND50 is typically 2 µg/mL.

Detection of MMP‑7 in Human Pancreas.

Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for MMP7 mRNA (ACD RNAScope Probe, catalog #488408; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human MMP7 polyclonal antibody (R&D Systems catalog # AF907) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands.

Applications for Human MMP‑7 Antibody

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑7 (Catalog # 907‑MP), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Crohn's stem cells

Western Blot

1 µg/mL
Sample: Capan‑1 human pancreatic adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line

Neutralization

Measured by its ability to neutralize Recombinant Human MMP‑7 (0.2 µg/mL, Catalog # 907-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 2 µg/mL.

Reviewed Applications

Read 1 review rated 4 using AF907 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal
alpha ‑defensin activation in innate host defense, releases tumor necrosis factor‑ alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Gene Symbol

MMP7

UniProt

P09237

Additional MMP-7 Products

Product Documents for Human MMP‑7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP‑7 Antibody

For research use only

Citations for Human MMP‑7 Antibody

Customer Reviews for Human MMP‑7 Antibody (1)

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  • Human MMP-7 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/09/2017
    This antibody was used to build an ELISA to measure MMP-7 in human plasma samples. The antibody worked as a matched pair with itself. i.e. is served as both the capture and detection molecule.
    Human MMP‑7 Antibody AF907

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