Human/Mouse Jak1 Antibody

Catalog # Availability Size / Price Qty
MAB42601
MAB42601-SP
Human/Mouse Jak1 Antibody in Western Blot
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Product Details
FAQs
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Reviews (3)

Human/Mouse Jak1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Jak1 in Western blots
Source
Monoclonal Mouse IgG2A Clone # 452804
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Jak1
Pro32-Phe286
Accession # P23458
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse Jak1 by Western Blot. View Larger

Detection of Human and Mouse Jak1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Jak1 Monoclonal Antibody (Catalog # MAB42601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jak1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jak1

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to IFN-alpha.

Long Name
Janus Kinase 1
Entrez Gene IDs
3716 (Human)
Alternate Names
EC 2.7.10; Jak1; JAK-1; JAK1Atyrosine-protein kinase JAK1; JAK1B; Janus kinase 1EC 2.7.10.2; JTK3

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Jak1 Antibody

Average Rating: 4.7 (Based on 3 Reviews)

5 Star
66.67%
4 Star
33.33%
3 Star
0%
2 Star
0%
1 Star
0%

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Human/Mouse Jak1 Antibody
By Anonymous on 02/19/2019
Application: WB Sample Tested: EMT6 cells Species: Mouse

Human/Mouse Jak1 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line Species: Human

Human/Mouse Jak1 Antibody
By Anonymous on 05/10/2017
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human