Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 452804
View all Jak1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Jak1
Pro32-Phe286
Accession # P23458

Specificity

Detects human and mouse Jak1 in Western blots

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse Jak1 Antibody

Detection of Human and Mouse Jak1 antibody by Western Blot.

Detection of Human and Mouse Jak1 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Jak1 Monoclonal Antibody (Catalog # MAB42601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Jak1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human/Mouse Jak1 by Simple WesternTM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Jak1 at approximately 139 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse Jak1 Monoclonal Antibody (Catalog # MAB42601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Jak1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Lysates of Daudi human Burkitt's lymphoma cell line.

Western Blot

1 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and LNCaP human prostate cancer cell line

Reviewed Applications

Read 4 reviews rated 4.8 using MAB42601 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jak1

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to IFN-alpha.

Long Name

Janus Kinase 1

Alternate Names

EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, Janus kinase 1EC 2.7.10.2, JTK3

Entrez Gene IDs

3716 (Human)

Gene Symbol

JAK1

UniProt

P23458

Additional Jak1 Products

Product Documents for Human/Mouse Jak1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Jak1 Antibody

This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.

For research use only

Citations for Human/Mouse Jak1 Antibody

Customer Reviews for Human/Mouse Jak1 Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse Jak1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hepa 1-6 mouse hepatoma cell line and B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 04/04/2020
    Human/Mouse Jak1 Antibody MAB42601
  • Human/Mouse Jak1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EMT6 cells
    Species: Mouse
    Verified Customer | Posted 02/19/2019
    Human/Mouse Jak1 Antibody MAB42601
  • Human/Mouse Jak1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line and Huh-7 human hepatoma cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human/Mouse Jak1 Antibody MAB42601
  • Human/Mouse Jak1 Antibody
    Name: Michelle Chen
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 05/10/2017

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