Human/Mouse/Rat EMAP-II Antibody

Catalog # Availability Size / Price Qty
MAB1910-100
MAB1910-SP
Detection of Human, Mouse, and Rat EMAP‑II by Western Blot.
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Human/Mouse/Rat EMAP-II Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human EMAP-II in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 959516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human EMAP-II
Ser147-Lys312
Accession # Q12904
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat EMAP-II antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat EMAP‑II by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, SK-OV-3 human ovarian adenocarcinoma cell line, M1 mouse myeloid leukemia cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat EMAP-II Monoclonal Antibody (Catalog # MAB1910) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMAP-II at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EMAP-II antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

EMAP‑II in HeLa Human Cell Line. EMAP-II was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/ Rat EMAP-II Monoclonal Antibody (Catalog # MAB1910) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry EMAP-II antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

EMAP‑II in Human Prostate Cancer Tissue. EMAP-II was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human/Mouse/Rat EMAP-II Monoclonal Antibody (Catalog # MAB1910) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMAP-II

EMAP-II is a pro-inflammatory cytokine that is chemotactic for monocytes and granulocytes. It is a cleavage product of multisynthetase complex auxiliary component p43.

Long Name
Endothelial-Monocyte Activating Polypeptide II
Entrez Gene IDs
9255 (Human); 13722 (Mouse); 114632 (Rat)
Alternate Names
AIMP1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; EMAP-2; EMAPII; EMAP-II; EMAPIImember 1 (endothelialmonocyte-activating); endothelial-monocyte activating polypeptide II; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43; SCYE1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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