Human/Mouse/Rat EMAP-II Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human EMAP-II in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 959516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human EMAP-II
    Ser147-Lys312
    Accession # Q12904
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat EMAP‑II by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, SK‑OV‑3 human ovarian adenocarcinoma cell line, M1 mouse myeloid leukemia cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat EMAP‑II Monoclonal Antibody (Catalog # MAB1910) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMAP‑II at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
EMAP‑II in HeLa Human Cell Line.
EMAP‑II was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/
Rat EMAP‑II Monoclonal Antibody (Catalog # MAB1910) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
EMAP‑II in Human Prostate Cancer Tissue. EMAP‑II was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human/Mouse/Rat EMAP‑II Monoclonal Antibody (Catalog # MAB1910) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMAP-II
EMAP-II is a pro-inflammatory cytokine that is chemotactic for monocytes and granulocytes. It is a cleavage product of multisynthetase complex auxiliary component p43.
  • Long Name:
    Endothelial-Monocyte Activating Polypeptide II
  • Entrez Gene IDs:
    9255 (Human); 13722 (Mouse); 114632 (Rat)
  • Alternate Names:
    AIMP1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; EMAP-2; EMAPII; EMAP-II; EMAPIImember 1 (endothelialmonocyte-activating); endothelial-monocyte activating polypeptide II; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43; SCYE1
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