GRB2 Antibody (481021)

Name: GRB2 Antibody (481021)
Brand: R&D Systems
SKU: MAB3846
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB3846

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 481021

View all GRB2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRB2
Met1-Val217
Accession # P62993

Specificity

Detects human, mouse and rat GRB2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for GRB2 Antibody (481021)

Detection of Human/Mouse/Rat GRB2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRB2 Monoclonal Antibody (Catalog # MAB3846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRB2 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for GRB2 Antibody (481021)

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line and NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: GRB2

GRB2 (growth factor receptor-bound protein 2), an adaptor protein involved in signal transduction, contains a central SH2 domain flanked by two SH3 domains. The SH2 domain binds to phosphotyrosine residues in RTKs such as PDGF and EGF, non-RTKs such as Bcr/Abl and FAK, and docking proteins such as FRS-2 and Gab1. The SH3 domains associate with proline rich motifs on the guanine nucleotide releasing factor, Sos, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathways. GRB2 also participates in the endocytosis of EGFR through its recruitment of Cbl. Tyrosine phosphorylation of GRB2 SH3 domains reduces binding to Sos and negatively regulates downstream signaling pathways including Ras, JNK and MAPK.