Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 883411
View all NKX2.2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NKX2.2
Met1-Lys128
Accession # O95096

Specificity

Detects human NKX2.2 in ELISA and Western Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat NKX2.2 Antibody

Detection of Human and Mouse NKX2.2 antibody by Western Blot.

Detection of Human and Mouse NKX2.2 by Western Blot.

Western blot shows lysates of A-673 human Ewing's sarcoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NKX2.2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NKX2 by Immunocytochemistry (ICC).2 antibody in A673 Human Cell Line by Immunocytochemistry (ICC).

NKX2.2 in A673 Human Cell Line.

NKX2.2 was detected in immersion fixed A673 human Ewing sarcoma cell line using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
NKX2 by Immunocytochemistry (ICC).2 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

NKX2.2 in Rat Cortical Stem Cells.

NKX2.2 was detected in immersion fixed rat cortical stem cells differentiated for 7 days using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human/Mouse/Rat NKX2.2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A637 human Ewing sarcoma cell line and rat cortical stem cells differentiated for 7 days

Western Blot

2 µg/mL
Sample: A‑673 human Ewing's sarcoma cell line and beta TC‑6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKX2.2

NKX2.2, also known as NKX2-2 or NKX2B, is an approximately 30 kDa, 273 amino acid homeobox nuclear transcription factor of the NKX2 protein family. It specifies differentiation of pancreatic islet cells such as alpha, beta, PP, and epsilon lineages.  It also functions in developmental neuronal patterning of the ventral spinal cord in response to graded Sonic Hedgehog gradients and contributes pathfinding of commissural neurons. Within the region used as an immunogen, human NKX2.2 shares 96% and 97% amino acid sequence identity with mouse and rat NKX2.2, respectively.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Entrez Gene IDs

4821 (Human); 18088 (Mouse); 366214 (Rat)

Gene Symbol

NKX2-2

UniProt

O95096 (Human)

Additional NKX2.2 Products

Product Documents for Human/Mouse/Rat NKX2.2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat NKX2.2 Antibody

For research use only

Citations for Human/Mouse/Rat NKX2.2 Antibody

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Protocols

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