Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody

Name: Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody
Brand: R&D Systems
SKU: MAB2998
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB2998

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Citations (3)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Capillary Immunoblot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 572106

View all PI 3-Kinase p85 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p85 alpha
aa Lys519-Gly644
Accession # P27986

Specificity

Detects human, mouse and rat PI 3-Kinase p85 alpha in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody

Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3-Kinase p85a Monoclonal Antibody (Catalog # MAB2998) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85a at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PI 3‑Kinase p85 alpha by Simple Western^TM.

Simple Western lane view shows lysates of A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PI 3‑Kinase p85 alpha at approximately 89 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3‑Kinase p85 alpha Monoclonal Antibody (Catalog # MAB2998). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: A172 human glioblastoma cell line

Western Blot

1 µg/mL
Sample: A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PI 3-Kinase p85 alpha

PI 3-Kinases phosphorylate the inositol ring on a variety of membrane-associated phosphatidylinositols. Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adaptor, coupling catalytic p110 to activated receptor tyrosine kinases.