TDP-43/TARDBP Antibody (671834)

R&D Systems | Catalog # MAB7778

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, In Situ Hybridization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 671834
View all TDP-43/TARDBP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TDP-43/TARDBP
Met1-Thr103
Accession # Q13148

Specificity

Detects human TDP-43/TARDBP in direct ELISAs. Detects human, mouse, and rat TDP-43/TARDBP in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TDP-43/TARDBP Antibody (671834)

Detection of Human TDP-43/TARDBP antibody by Western Blot.

Detection of Human TDP-43/TARDBP by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.4 µg/mL of Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse TDP-43/TARDBP antibody by Western Blot.

Detection of Human and Mouse TDP-43/TARDBP by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
TDP-43/TARDBP antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

TDP-43/TARDBP in A431 Human Cell Line.

TDP-43/TARDBP was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TDP-43/TARDBP antibody in Human Brain by Immunohistochemistry (IHC-P).

TDP-43/TARDBP in Human Brain.

TDP-43/TARDBP was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Rat TDP-43/TARDBP antibody by Simple WesternTM.

Detection of Human and Rat TDP-43/TARDBP by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for TDP-43/TARDBP at approximately 54 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Detection of Mouse TDP-43/TARDBP by Western Blot

Detection of Mouse TDP-43/TARDBP by Western Blot

FTLD‐related pathological changes in Tmem106b−/−Grn−/− miceA, BWestern blot analysis of p62, ubiquitin (Ub), TDP‐43 and p‐TDP-43 in RIPA‐ and urea‐soluble fractions from spinal cord (C5–C8) and brain of 5‐month‐old WT, Tmem106b−/−, Grn−/−, and Tmem106b−/−Grn−/− mice. Spinal cord: n = 3; brain: n = 4. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.C, DImmunostaining of p62 and ubiquitin in the spinal cord sections from 5‐month‐old WT, Tmem106b−/−, Grn−/−, and Tmem106b−/−Grn−/− mice. The number of p62 or Ub positive puncta was quantified. n = 3. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: ***P < 0.001, ****P < 0.0001. Scale bar = 10 μm.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32852886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of TDP-43/TARDBP by Immunoprecipitation.

Immunoprecipitation was performed on cell lysate of HAP1 human near-haploid cell line using 2.0 μg of Mouse Anti-Human TDP-43 Monoclonal Antibody (Catalog # MAB7778) pre-coupled to protein G or protein A beads. Immunoprecipitated TDP-43/TARDBP was detected with a Rabbit Anti-TDP-43 antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for TDP-43/TARDBP Antibody (671834)

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Immunoprecipitation

2 µg/1 mg cell lysate
Sample: Cell lysate of HAP1 human near-haploid cell line 

Simple Western

5 µg/mL
Sample: A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line

Western Blot

0.4-1 µg/mL
Sample: A431 human epithelial carcinoma cell line, NR8383 rat alveolar macrophage cell line, HeLa human cervical epithelial carcinoma cell line, and RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB7778 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TDP-43/TARDBP

TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amylotrophic lateral sclerosis and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates, along with a truncated 20-28 kDa form, in ubiquitin-containing inclusion bodies. TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis. Within aa 1-103, human and mouse TDP-43 share 100% aa sequence identity, and 99% aa identity with rat TDP-43.

Long Name

TAR DNA-binding Protein 43

Alternate Names

ALS10, TARDBP, TDP43

Entrez Gene IDs

23435 (Human)

Gene Symbol

TARDBP

UniProt

Q13148

Additional TDP-43/TARDBP Products

Product Documents for TDP-43/TARDBP Antibody (671834)

Certificate of Analysis

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Product Specific Notices for TDP-43/TARDBP Antibody (671834)

For research use only

Related Research Areas

Infectious Diseases

Citations for TDP-43/TARDBP Antibody (671834)

Customer Reviews for TDP-43/TARDBP Antibody (671834) (2)

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2 Customer Ratings
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  • Human/Mouse/Rat TDP-43/TARDBP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Muscle tissue
    Species: Human
    Verified Customer | Posted 09/07/2021
    TDP-43/TARDBP Antibody (671834) MAB7778
  • Human/Mouse/Rat TDP-43/TARDBP Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Paraffin section of human brain
    Species: Human brain cortex-formalin fixed paraffin embedded
    Verified Customer | Posted 10/25/2018
    Antigen retrieval performed-Heat mediated at pH9 Concentration: 1 in 100 overnight at 4C Secondary antibody: Alexafluor 594 anti-mouse antibody (Thermo) at 1 in 250 Primary and secondary antibodies prepared in Antibody Diluent/ background reducing from Agilent.
    TDP-43/TARDBP Antibody (671834) MAB7778

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Protocols

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