Human/Mouse/Rat TrkB Antibody

Catalog # Availability Size / Price Qty
MAB397-SP
MAB397-100
MAB397-500
Human/Mouse/Rat TrkB Antibody in Western Blot
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews

Human/Mouse/Rat TrkB Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TrkA, rhTrkC, or recombinant rat TrkA is observed.
Source
Monoclonal Mouse IgG2B Clone # 75133
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkB
Cys32-His430
Accession # Q16620
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat TrkB by Western Blot. View Larger

Detection of Human, Mouse, and Rat TrkB by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB397) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TrkB in Human Brain. View Larger

TrkB in Human Brain. TrkB was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB397) at 25 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

References
  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs
4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Product Datasheets

Citations for Human/Mouse/Rat TrkB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer's Disease
    Authors: ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye
    Cell Rep, 2019;28(3):655-669.e5.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Brain-derived neurotrophic factor (BDNF)-induced tropomyosin-related kinase B (Trk B) signaling is a potential therapeutic target for peritoneal carcinomatosis arising from colorectal cancer.
    Authors: Tanaka, Koji, Okugawa, Yoshinag, Toiyama, Yuji, Inoue, Yasuhiro, Saigusa, Susumu, Kawamura, Mikio, Araki, Toshimit, Uchida, Keiichi, Mohri, Yasuhiko, Kusunoki, Masato
    PLoS ONE, 2014;9(5):e96410.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
    Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
    Clin. Exp. Allergy, 2011;41(10):1392-1399.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Modulation of neurotrophin and neurotrophin receptor expression in nasal mucosa after nasal allergen provocation in allergic rhinitis.
    Authors: Raap U, Fokkens W, Bruder M, Hoogsteden H, Kapp A, Braunstahl GJ
    Allergy, 2008;63(4):468-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells.
    Authors: Gleissner CA, Zastrow A, Klingenberg R, Kluger MS, Konstandin M, Celik S, Haemmerling S, Shankar V, Giese T, Katus HA, Dengler TJ
    Eur. J. Immunol., 2007;37(1):177-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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