Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1034242
View all Neurogranin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Neurogranin
Met1-Asp78
Accession # Q92686

Specificity

Detects human Neurogranin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Neurogranin Antibody

Detection of Human, Mouse, and Rat Neurogranin by Western Blot.

Western blot shows lysates of human brain (motor cortex), mouse brain, and rat brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Neurogranin at approximately 14-15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Neurogranin in SW480 Human Cell Line.

Neurogranin was detected in immersion fixed SW480 human liposarcoma cell line (positive staining) and MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining is performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neurogranin in Human Brain (Cortex).

Neurogranin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human, Mouse, and Rat Neurogranin by Simple WesternTM.

Simple Western lane view shows lysates of human brain (motor cortex), mouse brain, and rat brain, loaded at 0.2 mg/mL. A specific band was detected for Neurogranin at approximately 12 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Applications for Human Neurogranin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SW480 human liposarcoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Simple Western

10 µg/mL
Sample: Human brain (motor cortex), mouse brain, and rat brain

Western Blot

2 µg/mL
Sample: Human brain (motor cortex), mouse brain, and rat brain

Reviewed Applications

Read 2 reviews rated 5 using MAB79471 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neurogranin

NRGN (Neurogranin/Ng; also known as RC3, p17 and BICKS) is a member of the neurogranin family of proteins. Although its predicted MW is 7.5 kDa, it runs anomalously at 15-19 kDa in SDS-PAGE. This is apparently due to the adoption of a rigid alpha -helical structure in a negatively charged medium. NRGN has limited expression, being found principally in excitatory neurons of the telencephalon, Golgi and Purkinje cells of the cerebellum, and platelets plus B and T cells in non-nervous tissue. Intracellularly, NRGN is found associated with membranes of the ER, Golgi and mitochondria. This association is often in the form of aggregates (or granules), thus giving rise to its name ("neuro-granules"). NRGN is also found in the nucleus and associated with the postsynaptic spines of dendrites. The principal function of NRGN appears to be the binding, sequestration and concentration of CaM (Calmodulin; a Ca-binding protein) in dendritic spines. Following NMDAR activation, Ca diffuses into the synaptic area, resulting in 1) the simply dissociation of CaM from NRGN, or 2) the phosphorylation of NRGN followed by its dissociation fro CaM. In either case, the freed CaM is now available to activate multiple downstream signaling pathways, some involved in LTP (or memory). Human NRGN is 78 amino acids (aa) in length. It contains one IQ domain (aa 26-47) that binds CaM, and a collagen-like region at the C-terminus (aa 48-78). Regulatory phosphorylation occurs on Ser36, and the N-terminal Met is acetylated. Full-length human NRGN shares 96% aa sequence identity with both mouse and rat NRGN.

Alternate Names

hng, NRGN, RC3

Entrez Gene IDs

4900 (Human); 64011 (Mouse); 64356 (Rat)

Gene Symbol

NRGN

UniProt

Q92686

Additional Neurogranin Products

Product Documents for Human Neurogranin Antibody

Certificate of Analysis

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Product Specific Notices for Human Neurogranin Antibody

For research use only

Customer Reviews for Human Neurogranin Antibody (2)

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2 Customer Ratings
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  • Human Neurogranin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 08/17/2022
    Human Neurogranin Antibody MAB79471
  • Human Neurogranin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human SW480 cell line
    Species: Human
    Verified Customer | Posted 01/05/2022
    Human Neurogranin Antibody MAB79471

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Protocols

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