Human Neurophysin II Antibody

Catalog # Availability Size / Price Qty
Neurophysin II in Human Brain Hippocampus Tissue.
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Human Neurophysin II Antibody Summary

Species Reactivity
Detects human Neurophysin II in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 62865
Protein A or G purified from ascites
Synthetic peptide containing human Neurophysin II
Accession # P01186
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Neurophysin II antibody in Human Brain Hippocampus Tissue by Immunohistochemistry (IHC-P). View Larger

Neurophysin II in Human Brain Hippocampus Tissue. Neurophysin II was detected in immersion fixed paraffin-embedded sections of human brain hippocampus tissue using Mouse Anti-Human Neurophysin II Monoclonal Antibody (Catalog # MAB009) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurophysin II

Arginine vasopressin (AVP)/Neurophysin 2 is a hormone synthesized in neurons in the hypothalamus that specifically binds vasopressin.  Vasopressin has a direct antidiuretic action on the kidney and causes vasoconstriction of the peripheral vessels.  Vasopressin regulates the tonicity of body fluid by causing the kidneys to reabsorb solute-free water and returning it to the circulation from the tubules of the nephron.  AVP released in high concentrations may also raise blood pressure by inducing vasoconstriction.  The stimulus for secretion of vasopressin is increased osmolality of the plasma which is monitored by the hypothalamus.  Vasopressin is often used to manage anti-diuretic hormone deficiency.

Entrez Gene IDs
551 (Human); 24221 (Rat)
Alternate Names
arginine vasopressin; ARVP; AVP; AVP-NPII; AVRP; neurohypophyseal; Neurophysin II; NP2; vasopressin-neurophysin 2-copeptin; VP

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Human Neurophysin II Antibody
By Anonymous on 05/30/2022
Application: IHC Sample Tested: Brain tissue Species: Human