Human Peptide YY Antibody

Catalog # Availability Size / Price Qty
MAB94562-100
MAB94562-SP
Human Peptide YY Antibody in ELISA
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Human Peptide YY Antibody Summary

Species Reactivity
Human
Specificity
Detects human Peptide YY in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1011014
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Peptide YY synthetic peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Peptide YY ELISA Standard Curve. Recombinant Human Peptide YY protein was serially diluted 2-fold and captured by Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peptide YY

Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.

Entrez Gene IDs
5697 (Human); 217212 (Mouse); 287730 (E. faecalis)
Alternate Names
Peptide tyrosine tyrosine; Peptide YY; Peptide-YY; PYY; PYY1; PYY-I

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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