Human Phospho-p38 alpha (T180/Y182) Antibody

Catalog # Availability Size / Price Qty
MAB8691
MAB8691-SP
Human Phospho-p38 alpha  (T180/Y182) Antibody in Data
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human Phospho-p38 alpha (T180/Y182) Antibody Summary

Species Reactivity
Human
Specificity
Detects human p38 alpha when phosphorylated at T180 and Y182.
Source
Monoclonal Mouse IgG2A Clone # 696602
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human p38 alpha T180/Y182 site
Accession # Q16539
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-p38a (T180/Y182) antibody by Western Blot. View Larger

Detection of Human Phospho-p38 alpha (T180/Y182) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-p38a (T180/Y182) Monoclonal Antibody (Catalog # MAB8691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-p38a (T180/Y182) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 alpha

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.

The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.

Entrez Gene IDs
1432 (Human); 26416 (Mouse); 81649 (Rat)
Alternate Names
MAPK14; p38 alpha; SAPK2a

Product Datasheets

Citations for Human Phospho-p38 alpha (T180/Y182) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro
    Authors: P Kanmani, A Ansari, J Villena, H Kim
    J Immunol Res, 2019;2019(0):3876896.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model
    Authors: X Lei, L Cai, X Li, H Xu, C Geng, C Wang
    Korean J. Physiol. Pharmacol., 2018;22(3):269-275.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The p38? Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1?
    Authors: S Simões-Sou, S Littler, SL Thompson, P Minshall, H Whalley, B Bakker, K Belkot, D Moralli, D Bronder, A Tighe, DCJ Spierings, N Bah, J Graham, L Nelson, CM Green, F Foijer, PA Townsend, SS Taylor
    Cell Rep, 2018;25(3):749-760.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. IL17A augments autophagy in Mycobacterium tuberculosis-infected monocytes from patients with active tuberculosis in association with the severity of the disease
    Authors: NL Tateosian, JM Pellegrini, NO Amiano, A Rolandelli, N Casco, DJ Palmero, MI Colombo, VE García
    Autophagy, 2017;0(0):14-Jan.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Regulatory module involving FGF13, miR-504, and p53 regulates ribosomal biogenesis and supports cancer cell survival
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Photoprotective Effects of Cycloheterophyllin against UVA-Induced Damage and Oxidative Stress in Human Dermal Fibroblasts
    PLoS ONE, 2016;11(9):e0161767.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-p38 alpha (T180/Y182) Antibody

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Human Phospho-p38 alpha (T180/Y182) Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human