Human Phospho-STAT3 (Y705) Antibody

Catalog # Availability Size / Price Qty
AF4607
AF4607-SP
Detection of Human Phospho-STAT3 (Y705) by Western Blot.
3 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (2)

Human Phospho-STAT3 (Y705) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT3 when phosphorylated at Y705.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human STAT3 Y705 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25-0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-STAT3 (Y705) antibody by Western Blot. View Larger

Detection of Human Phospho-STAT3 (Y705) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Phospho-STAT3 (Y705) antibody in IFN-alpha-treated Human Daudi Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-STAT3 (Y705) in IFN-alpha-treated Human Daudi Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (dark orange filled histogram) was stained with Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Immunocytochemistry STAT3 antibody in Daudi Human Burkitt's Lymphoma Cells by Immunocytochemistry (ICC). View Larger

STAT3 in Daudi Human Burkitt's Lymphoma Cells. Phospho-STAT3 was detected in immersion fixed IFN-alpha treated Daudi human Burkitt's lymphoma cell line using 10 µg/mL Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Long Name
Signal Transducer and Activator of Transcription 3
Entrez Gene IDs
6774 (Human); 20848 (Mouse); 25125 (Rat)
Alternate Names
Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

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Citations for Human Phospho-STAT3 (Y705) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Interleukin-6 Signaling in Triple Negative Breast Cancer Cells Elicits the Annexin A1/Formyl Peptide Receptor 1 Axis and Affects the Tumor Microenvironment
    Authors: L Vecchi, STS Mota, MAP Zóia, IC Martins, JB de Souza, TG Santos, AO Beserra, VP de Andrade, LR Goulart, TG Araújo
    Cells, 2022;11(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. LIMD2 Regulates Key Steps of Metastasis Cascade in Papillary Thyroid Cancer Cells via MAPK Crosstalk
    Authors: RP Araldi, TC de Melo, D Levy, DM de Souza, B Maurício, GA Colozza-Ga, SP Bydlowski, H Peng, FJ Rauscher, JM Cerutti
    Cells, 2020;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery
    Authors: W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu
    J. Exp. Clin. Cancer Res., 2019;38(1):62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Circular RNA circHIPK3 modulates autophagy via MIR124-3p-STAT3-PRKAA/AMPK? signaling in STK11 mutant lung cancer
    Authors: X Chen, R Mao, W Su, X Yang, Q Geng, C Guo, Z Wang, J Wang, LA Kresty, DG Beer, AC Chang, G Chen
    Autophagy, 2019;0(0):1-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Phospho-STAT3 (Y705) Antibody

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Human Phospho-STAT3 (Y705) Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Human Phospho-STAT3 (Y705) Antibody
By Anonymous on 01/17/2018
Application: IP Sample Tested: IPS2 induced pluripotent stem cells Species: Human