hh Human Podocalyxin Antibody MAB1658: R&D Systems

Human Podocalyxin Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 222328
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
    Ser23-Arg427
    Accession # AAB61574
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Podocalyxin
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podocalyxin
Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.
  • References:
    1. Li, J. et al. (2001) DNA Seq. 12:407.
    2. Hara, T. et al. (1999) Immunity 11:567.
  • Entrez Gene IDs:
    5420 (Human); 27205 (Mouse)
  • Alternate Names:
    GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Self-organization of the human embryo in the absence of maternal tissues
    Nat. Cell Biol., 2016;18(6):700-8.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  2. Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome.
    Authors: Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G
    PLoS ONE, 2013;8(10):e75945.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  3. Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury.
    Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P
    Stem Cells, 2012;30(8):1714-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
    Authors: Lasagni L, Ballerini L, Angelotti ML
    Stem Cells, 2010;28(9):1674-85.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Regeneration of glomerular podocytes by human renal progenitors.
    Authors: Ronconi E, Sagrinati C, Angelotti ML
    J. Am. Soc. Nephrol., 2009;20(2):322-32.
    Species: Human
    Sample Type: Whole Cells
    Application: cell sorting
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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

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Mouse IgG2A Antibody

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