Human Podocalyxin Antibody
Human Podocalyxin Antibody Summary
Ser23-Arg427
Accession # AAB61574
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
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Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podocalyxin
Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.
Product Datasheets
Citations for Human Podocalyxin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Identification of the SHREK family of proteins as broad-spectrum host antiviral factors
Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
bioRxiv : the preprint server for biology, 2021;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture -
Identification of the SHREK Family of Proteins as Broad-Spectrum Host Antiviral Factors
Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
Viruses, 2021;13(5):.
Species: Virus
Sample Types: Viral Particles
Applications: Immunoprecipitation -
Naive stem cell blastocyst model captures human embryo lineage segregation
Authors: A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo
Cell Stem Cell, 2021;28(6):1016-1022.e4.
Species: Human
Sample Types: Embryo
Applications: ICC -
Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
Science Advances, 2021;7(21):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis
Authors: R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe
Cell Stem Cell, 2021;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC -
Developmental potential of aneuploid human embryos cultured beyond implantation
Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
Nat Commun, 2020;11(1):3987.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Developmental potential of aneuploid human embryos cultured beyond implantation
Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
Nat Commun, 2020;11(1):3987.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
Oncotarget, 2017;8(59):99722-99739.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Self-organization of the human embryo in the absence of maternal tissues
Nat. Cell Biol., 2016;18(6):700-8.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome.
Authors: Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G
PLoS ONE, 2013;8(10):e75945.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury.
Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P
Stem Cells, 2012;30(8):1714-25.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
Authors: Lasagni L, Ballerini L, Angelotti ML
Stem Cells, 2010;28(9):1674-85.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Regeneration of glomerular podocytes by human renal progenitors.
Authors: Ronconi E, Sagrinati C, Angelotti ML
J. Am. Soc. Nephrol., 2009;20(2):322-32.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection
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