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Human/Porcine/Equine Integrin  beta 1/CD29 Antibody

Catalog #: MAB17783
2 Citations Datasheet / COA / SDS
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MAB17783
MAB17783-SP

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Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.
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Human/Porcine/Equine Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Human, Porcine, Equine
Specificity
Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 3, beta 4, beta 5, beta 8, recombinant mouse Integrin beta 1, beta 2, beta 6, or beta 7 is observed.
Source
Monoclonal Mouse IgG2B Clone # 419127
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1/CD29
Gln21-Asp728 (predicted)
Accession # P05556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Integrin  beta 1/CD29 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004), open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Porcine Mesenchymal Stem Cells antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Equine PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry Integrin beta 1/CD29 antibody in Porcine Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in Porcine Mesenchymal Stem Cells. Integrin beta 1/CD29 was detected in immersion fixed porcine mesenchymal stem cells using Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry View Larger

Integrin  beta 1/CD29 in A549 Human Cell Line. Integrin  beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Porcine/Equine Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta 1 subunit is 94% aa identical to pig and sheep Integrin beta 1 subunits.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

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Citations for Human/Porcine/Equine Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Purification of Pig Muscle Stem Cells Using Magnetic-Activated Cell Sorting (MACS) Based on the Expression of Cluster of Differentiation 29 (CD29)
    Authors: Kwang-Hwan Choi, Minsu Kim, Ji Won Yoon, Jinsol Jeong, Minkyung Ryu, Cheorun Jo et al.
    Food Science of Animal Resources
  2. Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture.
    Authors: Burton VJ, Butler LM, McGettrick HM, Stone PC, Jeffery HC, Savage CO, Rainger GE, Nash GB
    Exp. Cell Res., 2010-11-04;317(3):276-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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