Human/Porcine/Equine Integrin beta 1/CD29 Antibody

Name: Human/Porcine/Equine Integrin beta 1/CD29 Antibody
Brand: R&D Systems
SKU: MAB17783
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB17783

Submit a review

Citations (4)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Equine

Cited:

Human, Porcine

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 419127

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1/CD29
Gln21-Asp728 (predicted)
Accession # P05556

Specificity

Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 3, beta 4, beta 5, beta 8, recombinant mouse Integrin beta 1, beta 2, beta 6, or beta 7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Porcine/Equine Integrin beta 1/CD29 Antibody

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004), open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Detection of Integrin beta 1/CD29 in Porcine Mesenchymal Stem Cells by Flow Cytometry.

Porcine mesenchymal stem cells were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Integrin beta 1/CD29 in Equine PBMCs by Flow Cytometry.

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Integrin beta 1/CD29 in Porcine Mesenchymal Stem Cells.

Integrin beta 1/CD29 was detected in immersion fixed porcine mesenchymal stem cells using Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Integrin beta 1/CD29 in A549 Human Cell Line.

Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

View 2 more images

Applications for Human/Porcine/Equine Integrin beta 1/CD29 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs), porcine mesenchymal stem cells, and equine peripheral blood mononuclear cells (PBMCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line and porcine mesenchymal stem cells

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin beta 1/CD29 under non-reducing conditions only

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta 1 subunit is 94% aa identical to pig and sheep Integrin beta 1 subunits.