Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 3, beta 4, beta 5, beta 8, recombinant mouse Integrin beta 1, beta 2, beta 6, or beta 7 is observed.
Monoclonal Mouse IgG2B Clone # 419127
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1/CD29 Gln21-Asp728 (predicted) Accession # P05556
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Integrin beta 1/CD29 in Porcine Mesenchymal Stem Cells.
Integrin beta 1/CD29 was detected in immersion fixed porcine mesenchymal stem cells using Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta 1 subunit is 94% aa identical to pig and sheep Integrin beta 1 subunits.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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