Human/Porcine/Equine Integrin  beta 1/CD29 Antibody

(1 citations)   
  • Species Reactivity
    Human, Porcine, Equine
  • Specificity
    Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 3, beta 4, beta 5, beta 8, recombinant mouse Integrin beta 1, beta 2, beta 6, or beta 7 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 419127
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1/CD29
    Gln21-Asp728 (predicted)
    Accession # P05556
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Integrin  beta 1/CD29 under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of Integrin  beta 1/CD29 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Mouse Anti-Human/Porcine/Equine Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Integrin  beta 1/CD29 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
Integrin  beta 1/CD29 in Porcine Mesenchymal Stem Cells. Integrin  beta 1/CD29 was detected in immersion fixed porcine mesenchymal stem cells using Mouse Anti-Human/Porcine/Equine Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta 1 subunit is 94% aa identical to pig and sheep Integrin beta 1 subunits.
  • Entrez Gene IDs:
    3688 (Human); 16412 (Mouse)
  • Alternate Names:
    CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture.
    Authors: Burton VJ, Butler LM, McGettrick HM, Stone PC, Jeffery HC, Savage CO, Rainger GE, Nash GB
    Exp. Cell Res., 2011;317(3):276-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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