Key Product Details

Species Reactivity

Validated:

Human, Rat, Hamster

Cited:

Human, Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 535919
View all ACE-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1

Specificity

Detects human ACE-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Rat/Hamster ACE‑2 Antibody

Detection of Human ACE‑2 by Western Blot.

Western blot shows lysates of human kidney and human testis. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB9332) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of ACE-2 antibody in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP antibody by Flow Cytometry.

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9332) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.
ACE‑2 antibody in Rat Kidney by Immunohistochemistry (IHC-Fr).

ACE‑2 in Rat Kidney.

ACE‑2 was detected in immersion fixed frozen sections of rat kidney using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9332) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
ACE‑2 antibody in Hamster Lung by Immunohistochemistry (IHC-P).

ACE‑2 in Hamster Lung.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9332) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Human/Rat/Hamster ACE-2 Antibody by Western Blot

Detection of Human Human/Rat/Hamster ACE-2 Antibody by Western Blot

Sialic acid has modest effect on Spike binding and viral entry. (B) Western blot for purified Fc-proteins from HEK293T probed with anti-Fc, anti-RBD or anti-ACE2 Ab. CD44-Fc is positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33103998), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Rat/Hamster ACE‑2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP

Immunohistochemistry

1-25 µg/mL
Sample: Frozen rat kidney and paraffin-embedded hamster lung sections.

Western Blot

2 µg/mL
Sample: Human kidney and human testis

Reviewed Applications

Read 1 review rated 5 using MAB9332 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

References

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Gene Symbol

ACE2

UniProt

Q9BYF1

Additional ACE-2 Products

Product Documents for Human/Rat/Hamster ACE‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat/Hamster ACE‑2 Antibody

For research use only

Citations for Human/Rat/Hamster ACE‑2 Antibody

Customer Reviews for Human/Rat/Hamster ACE‑2 Antibody (1)

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  • Human/Rat/Hamster ACE-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Rat
    Verified Customer | Posted 09/10/2021
    Human/Rat/Hamster ACE‑2 Antibody MAB9332

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Protocols

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