Human/Rat PGD2 Synthase/PTGDS Antibody

Catalog # Availability Size / Price Qty
MAB10099-100
MAB10099-SP
Detection  of Human and Rat PGD2 Synthase/PTGDS by Western Blot.
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Human/Rat PGD2 Synthase/PTGDS Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat PGD2 Synthase/PTGDS in direct ELISA and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 997303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PGD2 Synthase/PTGDS
Met1-Gln190
Accession # P41222
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human and Rat PGD2 Synthase/PTGDS by Western Blot. View Larger

Detection of Human and Rat PGD2 Synthase/PTGDS by Western Blot. Western blot shows lysates of human heart tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PGD2 Synthase/PTGDS at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PGD2 Synthase/PTGDS  in Human Brain. View Larger

PGD2 Synthase/PTGDS in Human Brain. PGD2 Synthase/PTGDS was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGD2 Synthase/PTGDS

Prostaglandin-H2 D-isomerase or PGD2 synthase (PTGDS) is an enzyme that catalyzes the conversion of Prostaglandin H2 (PGH2) to Prostaglandin D2 (PGD2), a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Prostaglandin D2 synthase is used clinically as a diagnostic marker for Cerebrospinal fluid rhinorrhea, important in the assessment of head trauma severity. It has been also proposed that the urinary and serum levels pf PTGDS may be also a marker of renal damage in diabetes mellitus and hypertension.

Long Name
Prostaglandin D2 Synthase
Entrez Gene IDs
5730 (Human); 25526 (Rat)
Alternate Names
Beta-trace protein; Cerebrin-28; EC 5.3.99.2; Glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; Lipocalin-type prostaglandin-D synthase; L-PGDS; PDS; PGD2 Synthase; PGD2; PGDS2; PGDSLPGDS; PH2DISO; prostaglandin D synthase; prostaglandin D2 synthase (21kD, brain); prostaglandin D2 synthase 21kDa (brain); Prostaglandin-D2 synthase; prostaglandin-H2 D-isomerase; PTGDS

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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