Human Serum Albumin Antibody

Catalog # Availability Size / Price Qty
MAB14551-100
MAB14551-SP
Human Serum Albumin Antibody in ELISA
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Serum Albumin Antibody Summary

Species Reactivity
Human
Specificity
Detects Human Serum Albumin in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 188815
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Serum Albumin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB14552).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Serum Albumin DuoSet ELISA Kit (Catalog # DY1455) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Albumin ELISA Standard Curve. Recombinant Human Albumin protein was serially diluted 2-fold and captured by Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB14552) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB14551) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Albumin

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Entrez Gene IDs
213 (Human); 11657 (Mouse); 24186 (Rat)
Alternate Names
ALB; Albumin; cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20; PRO0883; PRO0903; PRO1341; serum albumin

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Serum Albumin Antibody

There are currently no reviews for this product. Be the first to review Human Serum Albumin Antibody and earn rewards!

Have you used Human Serum Albumin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review