Human TGF-alpha Antibody
Human TGF-alpha Antibody Summary
Val40-Ala89
Accession # P01135
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Applications
Human TGF-alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by TGF‑ alpha and Neutralization by Human TGF‑ alpha Antibody. Recombinant Human TGF-a (Catalog # 239-A) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TGF-a (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TGF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA). The ND50 is typically 0.15-0.75 µg/mL.
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TGF‑ alpha in Human Astrocytoma. TGF-a was detected in immersion fixed paraffin-embedded sections of human astrocytoma using 15 µg/mL Goat Anti-Human TGF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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TGF-alpha in Human Astrocytoma. TGF-a was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human TGF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-alpha
TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.
TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.
Product Datasheets
Citations for Human TGF-alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response
Authors: LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto
Sci Rep, 2019;9(1):8702.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015;194(3):1039-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
Authors: Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT
J. Invest. Dermatol., 2010;130(8):2031-40.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Association of tetraspanin CD9 with transmembrane TGFalpha confers alterations in cell-surface presentation of TGFalpha and cytoskeletal organization.
Authors: Imhof I, Gasper WJ, Derynck R
J. Cell. Sci., 2008;121(0):2265-74.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation -
The luteinizing hormone receptor-activated extracellularly regulated kinase-1/2 cascade stimulates epiregulin release from granulosa cells.
Authors: Andric N, Ascoli M,
Endocrinology, 2008;149(11):5549-56.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
Biol. Reprod., 2007;77(1):53-60.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
Authors: Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A
Eur. Respir. J., 2007;30(6):1064-73.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
Cell, 2006;124(6):1225-39.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
J. Invest. Dermatol., 2006;126(9):2096-105.
Species: Human
Sample Types: Serum
Applications: Immunodepletion -
Mek1 alters epidermal growth and differentiation.
Authors: Scholl FA, Dumesic PA
Cancer Res., 2004;64(17):6035-40.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Dev. Biol., 2004;266(2):223-37.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
Authors: Kansra S, Johnson JL
Mol. Biol. Cell, 2004;15(9):4299-309.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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