Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Porcine

Applications

Validated:

Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Blocking, ELISA Development, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 383936
View all TLR2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TLR2
Accession # O60603

Specificity

Stains human TLR2 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TLR2 Antibody

IL‑8 Secretion Induced by Pam3CSK4and Neutralization by Human TLR2 Antibody.

IL‑8 Secretion Induced by Pam3CSK4and Neutralization by Human TLR2 Antibody.

The synthetic tripalmitoylated lipopeptide Pam3CSK4stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). IL-8 secretion elicited by Pam3CSK4(0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND50 is typically 0.03-0.15 µg/mL.
Detection of TLR2 antibody in Human Monocytes antibody by Flow Cytometry.

Detection of TLR2 in Human Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human TLR2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Neutralization

Measured by its ability to neutralize Pam3CSK4-induced IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 0.5 µg/mL The synthetic tripalmitoylated lipopeptide Pam3CSK4.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB2616 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

References

  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
  3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  4. Flo, T. et al. (2001) J. Leukoc. Biol. 69:474.
  5. Akira, S. (2003) Curr. Opin. Immunol. 15:5.
  6. Yamamoto, M. et al. (2002) Nature 420:324.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Entrez Gene IDs

7097 (Human); 24088 (Mouse); 310553 (Rat)

Gene Symbol

TLR2

UniProt

O60603

Additional TLR2 Products

Product Documents for Human TLR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TLR2 Antibody

For research use only

Citations for Human TLR2 Antibody

Customer Reviews for Human TLR2 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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2 Stars
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1 Stars
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Showing  1 - 3 of 3 reviews Showing All
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  • Human TLR2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/13/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human TLR2 Antibody MAB2616
  • Human TLR2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
  • Human TLR2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: KF6 human keratinocytes and THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 04/26/2016
    Human TLR2 Antibody MAB2616

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