Human TLR2 Antibody

(16 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Stains human TLR2 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 383936
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human TLR2
    Accession # O60603
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize Pam3CSK4-induced IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 0.5 µg/mL The synthetic tripalmitoylated lipopeptide Pam3CSK4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑8 Secretion Induced by Pam3CSK4 and Neutralization by Human TLR2 Antibody. The synthetic tripalmitoylated lipopeptide Pam3CSK4 stimulates IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL‑8 Quantikine ELISA Kit (Catalog # D8000C). IL‑8 secretion elicited by Pam3CSK4 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND50 is typically 0.03-0.15 µg/mL.
Detection of TLR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

  • References:
    1. Wetzler, L. (2003) Vaccine 21:S2/55.
    2. Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
    3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
    4. Flo, T. et al. (2001) J. Leukoc. Biol. 69:474.
    5. Akira, S. (2003) Curr. Opin. Immunol. 15:5.
    6. Yamamoto, M. et al. (2002) Nature 420:324.
  • Long Name:
    Toll-like Receptor 2
  • Entrez Gene IDs:
    7097 (Human); 24088 (Mouse); 310553 (Rat)
  • Alternate Names:
    CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CLEC5A is a critical receptor in innate immunity against Listeria infection
    Authors: ST Chen, FJ Li, TY Hsu, SM Liang, YC Yeh, WY Liao, TY Chou, NJ Chen, M Hsiao, WB Yang, SL Hsieh
    Nat Commun, 2017;8(1):299.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Specific pathways mediating inflammasome activation by Candida parapsilosis
    Authors: A Tóth, E Zajta, K Csonka, C Vágvölgyi, MG Netea, A Gácser
    Sci Rep, 2017;7(0):43129.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
    Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
    J. Immunol., 2017;0(0):.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Functional Assay
  4. TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation
    PLoS ONE, 2016;11(8):e0161428.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. A Model to Explain How the Bacille Calmette Gu�rin (BCG) Vaccine Drives Interleukin-12 Production in Neonates
    PLoS ONE, 2016;11(8):e0162148.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016;11(11):e0167410.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.
    Authors: Brummelman J, Veerman R, Hamstra H, Deuss A, Schuijt T, Sloots A, Kuipers B, van Els C, van der Ley P, Mooi F, Han W, Pinelli E
    Infect Immun, 2015;83(1):227-38.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  9. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015;22(7):1219-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation.
    Authors: Park S, Lee S, Kim H, Lee W, Hong K, Kim C
    Eur J Immunol, 2015;45(4):1216-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
    Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M
    J Biol Chem, 2014;289(16):11068-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro.
    Authors: Li D, Wang X, Wu J, Quan W, Ma L, Yang F, Wu K, Wan H
    PLoS ONE, 2013;8(2):e56616.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
    Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
    Innate Immun, 2012;18(3):531-40.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
    Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
    Br. J. Dermatol., 2011;165(2):302-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
    Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
    Clin. Exp. Immunol., 2009;156(2):285-93.
    Species: Human
    Sample Type: Saliva
    Application: ELISA Development
Expand to show all 16 Citations
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human TLR2 Antibody MAB2616
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 Flow Human Anonymous 04/26/2016
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Flow Cytometry Human TLR2 Antibody MAB2616
Flow Cytometry: Human TLR2 Antibody [MAB2616]

Summary

ApplicationFlow Cytometry
Sample TestedKF6 human keratinocytes,THP-1 human acute monocytic leukemia cell line
SpeciesHuman

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