Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1074702
View all TOMM20 Primary Antibodies »

Product Specifications

Immunogen

TOMM20 containing synthetic peptide
Accession # Q15388

Specificity

Detects a synthetic peptide specific for human TOMM20 containing amino acid 55 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TOMM20 Antibody

Detection of TOMM20 in A549 Human Cell Line.

TOMM20 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the mitochondrion membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human TOMM20 by Western Blot.

Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TOMM20 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of TOMM20 in Human Lung.

TOMM20 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of bronchial epithelium. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of TOMM20 in Human Testis.

TOMM20 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial and Leydig cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TOMM20 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung and testis

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TOMM20

TOMM20 (Translocase of Outer Mitochondrial Membrane 20) is a receptor and key unit of the TOM complex.  The TOM complex translocates proteins produced from nuclear DNA through the mitochondrial membrane for use in oxidative phosphorylation.  TOMM20 is a conserved outer membrane protein and depletion of TOMM20 produces a large number of dysfunctional mitochondria with the loss of membrane potential.  Increased TOMM20 expression has been associated with malignancy of various cancers and contributes to tumorigenesis.

References

  1. Park SH, Lee AR, Choi K, Joung S, Yoon JB, Kim S. TOMM20 as a potential therapeutic target of colorectal cancer. BMB Rep. 2019 Dec;52(12):712-717. doi: 10.5483/BMBRep.2019.52.12.249. PMID: 31818360; PMCID: PMC6941759.Nijenjuis M., Hämmerling GJ.  J Immunol. 1996 Dec 15;157(12):5467-77.
  2. Yin L, Ye Y, Zou L, Lin J, Dai Y, Fu Y, Liu Y, Peng Y, Gao Y, Fu Y, Qi X, Deng T, Zhang S, Li X. AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. J Exp Clin Cancer Res. 2023 Aug 10;42(1):204. doi: 10.1186/s13046-023-02776-0. PMID: 37563661; PMCID: PMC10413764.

Long Name

TOMM20

Alternate Names

MAS20, mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolo, MOM19, outer mitochondrial membrane receptor Tom20, TOM20, translocase of outer mitochondrial membrane 20 hom

Entrez Gene IDs

9804 (Human); 67952 (Mouse); 266601 (Rat)

Gene Symbol

Q15388

UniProt

Q15388 (Human)

Additional TOMM20 Products

Product Documents for Human TOMM20 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TOMM20 Antibody

For research use only

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Protocols

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