Human UCH-L1/PGP9.5 Antibody

Catalog # Availability Size / Price Qty
MAB6007
MAB6007-SP
Human UCH-L1/PGP9.5 Antibody in Data
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Product Details
Citations (1)
FAQs
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Human UCH-L1/PGP9.5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human UCH-L1/PGP9.5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UCH-L3 is observed.
Source
Monoclonal Mouse IgG2A Clone # 671108
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human UCH-L1/PGP9.5
Gln2-Ala223
Accession # P09936
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant Human UCH-L1/PGP9.5 (Catalog # 6007-CY), see our available Western blot detection antibodies
Knockout Validated
UCH-L1/PGP9.5 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in UCH-L1/PGP9.5 knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human UCH-L1/PGP9.5 antibody by Western Blot. View Larger

Detection of Human UCH-L1/PGP9.5 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCH-L1/PGP9.5 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry UCH-L1/PGP9 by Immunohistochemistry (IHC-P).5 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

UCH-L1/PGP9.5 in Human Prostate. UCH-L1/PGP9.5 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse UCH-L1/PGP9.5 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse UCH-L1/PGP9.5 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cortex) tissue and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for UCH-L1/PGP9.5 at approximately 32 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human UCH-L1/PGP9.5 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human UCH-L1/PGP9.5 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCH-L1/PGP9.5

UCH-L1 (ubiquitin carboxyterminal hydrolase isozyme 1; also PGP9.5) is a 24-27 kDa member of the peptidase C12 family of enzymes. It shows restricted expression, being found in neurons and oocytes. UCH-L1 has dual enzymatic activity. As a monomer, it is a ubiquitin hydrolase that removes ubiquitin from modified proteins; as a homodimer, it acts as a ligase that creates ubiquitin dimers. In neurons, UCH-L1’s most important role appears to be that of generating free ubiquitin. Human UCH-L1 is 223 amino acids (aa) in length. It is O-glycosylated, ubiquitinated, and farnesylated; when farnesylated, it becomes associated with cell membranes. Three potential splice forms are reported. One shows a two aa substitution for aa 12-15, a second contains an alternative start site at Met82, and a third shows the same start site coupled with a deletion of aa 138-153. Full-length human UCH-L1 shares 95% aa identity with mouse UCH-L1.       

Long Name
Ubiquitin C-terminal Hydrolase L1
Entrez Gene IDs
7345 (Human); 22223 (Mouse)
Alternate Names
EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1

Product Datasheets

Citation for Human UCH-L1/PGP9.5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) Promotes Uterine Serous Cancer Cell Proliferation and Cell Cycle Progression
    Authors: SY Kwan, CL Au-Yeung, TL Yeung, A Rynne-Vida, KK Wong, JI Risinger, HK Lin, RE Schmandt, MS Yates, SC Mok, KH Lu
    Cancers (Basel), 2020;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IP

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human UCH-L1/PGP9.5 Antibody

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Human UCH-L1/PGP9.5 Antibody
By Anonymous on 05/21/2021
Application: ICC/IF Sample Tested: Skin tissue Species: Human

1st PGP9.5 in 1:500, 2nd antibody Cy3
Red staining = laminin + Cy5
+Dapi mounting medium

Collagen autofluorescence was a problem in some of the sections.
Zamboni Fixed, 20% sucr.