Detection of Human Urocortin by Western Blot.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Urocortin at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Urocortin in Human Brain.
Urocortin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
UCN1) is a member of the widely expressed corticotropin releasing factor (CRF)
peptide family that includes UCN1-3 and CRF. UCN stimulates cAMP and MAPK
pathways via either of two G-protein coupled receptors, CRFR1 and CRFR2, while
other family members prefer CRFR1 (CRF) or CRFR2 (UCN2, UCN3). In the brain,
UCN has been implicated in counteracting stress-provoked anxiety and
suppressing appetite. It also modulates metabolic activity in peripheral
tissues and protects the heart from ischemia/reprofusion injury. The UCN cDNA
encodes 124 aa, including a 25 aa signal sequence, a 57 aa propeptide, and a 42
aa mature active 5 kDa UCN peptide. The proform of human UCN (aa 26-124, 11 kDa predicted) shares
approximately 80% aa sequence identity with mouse and rat UCN.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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